BEAUTY – Thot Nutrition – Power of Essential Amino Acids

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The Breakthrough

There are moments in supplement formulation when a supplement is more than a supplement. It is a breakthrough. THOT MAG-B-NOX is one of those moments. It is a game-changer.

THOT MAG-B-NOX is created to activate your body’s natural Nitric Oxide (NO) production in a more complex way that you have experienced so far. This formulation is based on a very deep understanding of how NO is formed and utilized by the body and of what the body actually needs in order to make it happen flawlessly.

This supplement is more than just another NO booster. It is designed for both rapid effect and long-term optimization of the entire body. It doesn’t just focus on NO production; it takes care of the pathways that produce it and it makes sure to keep them healthy. How? Through all the ingredients that it contains. This is a formula like no other.

Dysfunctions associated with NO deficiency

Erectile dysfunctions
High blood pressure (NO ensures dilatation of the endothelial layer of the blood vessel – the firs layer; means more blood is circulating and the blood vessels are elastic
Metabolic diseases and diabetes – NO is required for insulin signaling; If you cannot produce NO, you become insulin resistance and diabetic
Exercise resistance and stamina. You do not have power to exercise, you become rapidly “out of breath”. Try to walk the stairs and see how you perform. Usually is because you are NO deficient
Alzheimer’s disease because you have low flow of blood to the brain and become insulin resistance in the brain – Type 3 diabetes. NO is correcting everything we know about Alzheimer. NO inhibits the oxidative stress associated with Alzheimer’s disease, preventing the accumulation of amyloid plaque and the presence of Tau protein (inflammatory marker).
Inflammation – NO reduce inflammation in the body and in the brain.
Immune disfunction – NO prevents immune dysfunctions. NO assures that nutrients flow to the cells and take away the garbage. In this way you have no miss-folding proteins
Oxidative stress related diseases and Immune dysfunctions.
Miss-folded protein dysfunctions. NO assures that nutrients flow to the cells and take away the garbage. In this way you have no miss-folding proteins
Nitric oxide (NO) plays a complex role in Alzheimer’s disease, contributing to both neuro-protective and neurotoxic processes
Nitric oxide (NO) may support neuronal function by enhancing neuronal excitability and promoting synaptic plasticity.

Longevity associated diseases. NO is helping the 3 most important factors for anti-aging and longevity:

NO(Nitric Oxide) gives stem cells the signal to mobilize; thus, stimulates and mobilize stem cells, especially plumy-potent stem cells
Telomere extension. NO activate the enzyme telomerase that prevents telomeres shortening. It signals the production of the telomerase enzymes and regulates the proper function of the telomerase enzyme.
Mitochondrial functions. NO gives the signal to the cell that the body needs more mitochondria and the body needs for the existing mitochondria to function at the best possible function, to produce as much cellular energy as possible with less oxygen.

Chronic Diseases Associated with NO Deficiency

Non- healing wounds. Scars, necrotic wounds, etc.
Retinopathy
Diabetic ulcers and necrosis
Macular degeneration
Degenerative pancreatitis

What is in the vial? Yes, it it in liquid form now.

Easier to take, faster action time,

In just one or two daily shots, THOT MAG-B-NOX delivers:

6 amino acids (3000 mg total)( L-Arginine (precursor for BPC157 Peptide), L-Citrulline, L-Lysine HCl, L-Leucine, L-Tryptophan, L-Methionine)
5 types of Magnesium (900 mg total) (Magnesium bisglycinate, Magnesium malate, Magnesium taurate, Magnesium citrate (magnesium salts of citric acid), Magnesium ascorbate)
5 powerful plant-based NO activators (Hawthorn Berries Extract 5:1, Açaí Berries Extract 10:1, Aronia Berries Extract 10:1, Moringa Leaf Extract 10:1, Red Spinach Extract (Oxystorm®)
Non-acidic Vitamin C (from Magnesium ascorbate)
Essential electrolytes (Na, K, Cl) (from NaCl and Potassium citrate)
B vitamins for mitochondrial support (Vitamin B3 – Niacin, Vitamin B6 – Pyridoxine HCL, Vitamin B12 – Methycobalamin)

Now… why choose Thot MAG-B-NOX when choosing a Nitric Oxide (NO) booster supplement?

There are so many reasons! But let us give you the most important ones:

It is a liquid form supplement!
- This means that you are done with swallowing tons of tablets. Until now, you had to take multiple tablets from multiple products to make sure you had all the ingredients in the right quantities to boost your NO production and to make sure that the mechanisms that produce it stay healthy. Now you have them all in one shot.
- This also means rapid absorption and immediate impact. While with tablets and capsules you need to wait for them to dissolve, a liquid shot provides you the ingredients ready for absorption and utilization.
It covers ALL the pathways involved in NO production and utilization. This supplement offers:
- the ingredients that stimulate NO production
- the ingredients that are precursors for NO
- the ingredients that optimize NO synthesis
- the ingredients that protect and nurture the pathways involved in NO synthesis
- the ingredients that help the body utilize NO properly

If this introduction got your attention, feel free to try our product right now.

THOT MAG-B-NOX: The energy makeover booster

SHOP MAG-B-NOX

If you’re not that sure yet or if you simply want to know what is Nitric Oxide (NO), how it benefits your health and to understand the science behind our formula continue reading this article.

Nitric Oxide – What It Is and How It Helps?

Nitric Oxide (NO) is a critical signaling molecule that influences nearly every system in the human body, and it is produced naturally by your vascular endothelium (the inner layer of your blood vessels).

In short, NO is critical in:

Vasodilation (relaxation of blood vessels)
Oxygen and nutrient delivery to muscles and tissues
Blood pressure regulation
Mitochondrial respiration and function
Regeneration and antiaging
Tissue healing and regeneration
Strem cells activation and coordination
Telomers expression
Neurotransmission
Immune defense mechanisms
Sexual function and libido
And much more…

Due to its implications in every aspect of human physiology, NO got the attention of medical doctors and scientists and there are a few of them who studied this tiny gas molecule in depth.

For decades, nitric oxide (NO) was considered just a toxic air pollutant. That belief changed completely in the late 20th century. In 1998, the Nobel Prize in Physiology or Medicine was awarded to Robert F. Furchgott, Louis J. Ignarro, and Ferid Murad^{^[1]} for their discovery that nitric oxide is a signaling molecule in the cardiovascular system^{^[2]}.

Their work proved that nitric oxide is produced naturally by the human body, essential for vasodilation (blood vessel relaxation) and critical for blood pressure regulation, blood flow, and cellular communication.

This discovery revolutionized medicine and laid the foundation for modern understanding of cardiovascular health, erectile function, metabolic regulation, immune signaling and more. Nitric oxide went from being seen as a “toxic gas” to being recognized as a fundamental molecule of life.

Decades later, impressed by this discovery and forced by personal circumstances involving health problems that needed a different approach, Dr. Nathan Bryan continued to study this molecule. Today, we are grateful for the work he has done and for the knowledge that he has shared about this miracle gas molecule.

Dr. Nathan Bryan is an American biochemist and nitric oxide (NO) researcher for over 18–20 years. He worked at Baylor College of Medicine, published dozens of scientific articles and edited medical books on the topic of NO^[3]. Dr. Nathan S. Bryan is considered one of the world’s leading experts on nitric oxide.

With aging, stress, inflammation, poor diet, and sedentary lifestyles, NO production declines over time. This often happens silently and people notice it only when symptoms appear, symptoms like fatigue, cold hands and feet, headaches, muscle tightness, slower recovery, poor focus, sexual dysfunction etc.

Although NO is known in the sports industry and supplements are used for athletic performance for a while now, a proper level of NO in the body helps ordinary people as well. We are talking about the quality of your life: how fast your brain thinks, how well your blood flows, how deeply you sleep, how alive you feel.

Nitric Oxide — How It’s Made and How It Works?

Nitric oxide (NO) is a microscopic gas, yet it controls the fundamental aspect of your biology.

There are two major pathways NO is produced:

1. The first pathway is Arginine → NO (NOS) enzymatic pathway

NO is created in the body, from L‑arginine in the inner layer of your blood vessels – the endothelium. The body needs the eNOS enzyme to create NO. NO signals your vessels to relax and open. Studies dating back decades confirm that human endothelial cells synthesize NO directly from L‑arginine—and that providing more arginine boosts NO availability and improves vascular function^[4].
This pathway depends on: L-arginine, L-citrulline, cofactors (BH4, vitamins), functional NOS enzyme. If the environment of your cells had the right support, with nutrients like magnesium, vitamin C, and B‑vitamins, NO would flow flawlessly. But modern stress, inflammation, poor nutrition, and aging suppress slows down the production of NO and disrupt its function.
The main problem that may occur in this pathway is the “uncoupling” of the enzyme NOS. As a result, instead of NO it produces free radicals (superoxide). That is why classic arginine supplements may not work and this is the reason we packed the formula with powerful anti-oxidants.

2. The second pathway it the Nitrate–nitrite–NO pathway (non-enzymatic)

Thankfully, there’s a secondary route that helps the NO pathway: nitrate→nitrite→NO. Dietary nitrates from some plants and leafy greens convert to nitrite via oral bacteria and then into NO in the bloodstream.
So, if one pathway doesn’t work, we have the other. This last one is clinically validated and especially effective when endogenous synthesis (the first described pathway) is compromised^[5]..
This pathway depends heavily on oral microbiome (beneficial bacteria on tongue, gums) and gastric pH (sufficient acidity)
The main problem that may occur is usually due to antibacterial mouthwash! It destroys NO-producing bacteria, which usually increases blood pressure as a result. So, there you have it – one information that you may not have known until now: toothpaste + fluoridated water can alter oral microbiota and enzymes.

MAG-B-NOX supports both pathways.

Once produced in the endothelial cells, NO activates guanylate cyclase, raising cGMP. This helps to relax the vascular smooth muscle, lower the blood pressure, improve the microcirculation and prevent platelet aggregation. That was proven by Nobel prize–winning work identifying NO as the “endothelium-derived relaxing factor”^[6].

NO is ultra-reactive and very diffusible so it gets very quickly into the nearby cells. NO is a signaling molecule that affects nearly every organ system.

These are the main benefits for your body, and this is how they take place.

In your cardiovascular system, NO keeps vessels pliable, reduces stiffness, supports angiogenesis (new capillaries), and guards against clots and hypertension
In your muscles, NO improves mitochondrial respiration and oxygen delivery during movement, reducing oxygen cost of exercise and boosting endurance. Numerous clinical trials—especially using beetroot juice—report higher reps and power output at lower oxygen cost^[7].
In your brain, NO functions as a neurotransmitter. It supports memory consolidation, neuroplasticity, learning, and mood regulation^[8] ^[9].
NO also plays an essential role in your immune system. It helps macrophages and T‑cells identify pathogens and modulates inflammatory signaling —allowing healing without excessive immune overreaction.
On a cellular level NO has direct effects on stem cells activation and functions, telomeres expressions and mitochondria functions – the primary mechanisms of antiaging and longevity.
On a metabolic level, NO enhances insulin sensitivity, supports glucose uptake into muscle cells, and reduces inflammatory markers. Clinical reviews highlight nitrate supplementation as a strategy to improve metabolic health and decrease cardiometabolic risk^[10] ^[11].
And it even touches sexual health – because NO delivers the blood flow that triggers arousal, lubrication, and orgasm. Without it, desire may exist, but the body stays unresponsive.

When NO is low, every system slows down.

You feel fatigue, brain fog, poor recovery, low libido, cold extremities, poor circulation, and slow healing. Often dismissed as “aging,” the root cause is frequently NO deficiency.

Restoring NO transforms everything.

Vessels relax, mitochondria regenerate, circulation wakes, cognitive clarity returns, sleep deepens, immunity regulates, and your system begins to recover on its own.

This is why we created THOT MAG‑B-NOX.

It activates both NO pathways – providing L‑arginine, L‑citrulline and nutrients that support both the eNOS pathway and the nitrate‑nitrite‑NO pathway. It also has antioxidant protection to preserve NO and regeneration boosters to support longevity. The result? THOT MAG-B-NOX doesn’t simply increase NO. It creates an entire ecosystem for sustained NO synthesis, retention and function.

Diseases & Dysfunctions associated with low Nitric Oxide (NO)

Without NO you have no vasodilation, so no increased blood flow – this is the basis of erection, normal blood pressure, brain irrigation and physical performance.

The most important diseases and dysfunctions associated with NO deficiency:

Erectile dysfunction – this is the first sign of NO deficiency and accelerated cardiovascular disease^[12] – this is a very underrated and underdiagnosed condition, and this is because its intimate nature. Who would like to admit that they have erectile dysfunction? But behind closed doors, people know they have a problem. This is the reality and it affects both men and women. No NO means no vasodilation which means no arousal.
High blood pressure – because there is no more NO-mediated vasodilation, the same volume of blood is forced to passes through narrower arteries. This is the mechanism that causes bold pressure to increase^[13]. It is just physics.
Metabolic diseases and diabetes – NO production is necessary for insulin signaling; without NO, the cell develops insulin resistance and diabetes occurs^[14].
Antiaging and regeneration – NO has direct effects on stem cells, telomeres and mitochondria – the primary mechanisms of antiaging and longevity.
Exercise intolerance – if you can’t climb a flight of stairs or can’t sustain 15–30 min of moderate exercise or you don’t have power to exercise and you become rapidly “out of breath”, you are most likely deficient in NO^[15].
Alzheimer’s disease – this is vascular disease (low blood flow) combined with an insulin resistance in the brain. It is also called “type 3 diabetes^[16]. NO is correcting everything we know about Alzheimer. NO inhibits the oxidative stress associated with Alzheimer’s disease, preventing the accumulation of amyloid plaque and the presence of Tau protein (inflammatory marker).
Oxidative stress & immune dysfunction – without NO you no longer have good nutrient transport, and waste is no longer cleaned up. This leads to misfolded proteins and chronic inflammation^[17]. NO assures that nutrients flow to the cells and it helps blood to take away the garbage. In this way, you have no miss-folding proteins.
Chronic wounds, ulcers, necrosis^{^[18]} – diabetic ulcers and necrosis, macular degeneration, retinopathy, degenerative pancreatitis, non-healing wounds, escares and more – they are correlated with microcirculation and endothelial dysfunction; mechanistically it ties in perfectly with NO deficiency.

Nitric Oxide (NO) and LONGEVITY – key points

According to science, endothelial nitric oxide (NO) production and bioavailability are highest in young, healthy individuals and decline progressively with aging^[19]. It is believed that up to ~20 years NO production is optimal.

NO-dependent endothelial vasodilation declines significantly by early to mid-adulthood, resulting in reduced NO production and increased oxidative stress^[20], so that approximately by age 40, the body generally loses 40-60% of the ability to produce NO.

After midlife (~40), nitric oxide bioavailability declines progressively with each decade, largely due to oxidative stress, eNOS uncoupling, and impaired endothelial signaling^[21]. It is believed that the rate of decline is 10–12% per decade.

Advanced aging (~70-80 years) is associated with a profound reduction in nitric oxide–mediated vasodilation, with 70–75% less compared with young adults^[22].

Poor lifestyle factors accelerate endothelial dysfunction and nitric oxide deficiency, leading to early onset hypertension, insulin resistance, erectile dysfunction and vascular disease — even in adolescents and young adults^[23].

Now that we have seen how NO declines with age, it is obvious that, to benefit longer from its effects, you must do certain things to stimulate the body to produce more NO for a longer period. Just like when it was when you were younger.

If you manage to stop the decline and produce more NO, it is going to help you not only to prolong your life, but also to significantly increase your life quality.

How does NO do this? Well, NO has direct effects on stem cells, telomeres and mitochondria – the primary mechanisms of antiaging and longevity.

Nitric oxide is a key signaling molecule involved in the mobilization, recruitment, and function of stem cells^[24]. It mobilizes them into the tissues where they are needed – eNOS-derived NO is required for stem cell mobilization from bone marrow and this proves that NO bioavailability is directly linked to regenerative capacity. Without sufficient NO, regeneration is slow, wounds and injuries are difficult to heal^[25].
NO can activate telomerase, the enzyme that is responsible for maintaining telomere length^[26]. Sustained NO production contributes to telomere protection and is associated with longevity^[27].
Nitric oxide regulates mitochondrial respiration and oxygen utilization, directly modulating mitochondrial oxygen consumption^[28]. At physiological levels, NO improves mitochondrial efficiency – it enhances mitochondrial coupling efficiency, reduces electron leakage, and optimizes ATP production. So we have more ATP with less O2^[29]. NO deficiency, on the other hand, leads to mitochondrial dysfunction and high oxidative stress, leading to cellular disfunction and metabolic decline. This feature is associated with “sherpa gene”; a genetic unique characteristic that allow some native population to function and thrive at high altitude where oxygen has lower concentration.

Knowing that these 3 mechanisms that are the core pillars in regeneration and longevity need proper amounts of Nitric Oxide to function properly, it is only natural to do our best to enhance and optimize NO levels in our bodies for as long as possible.

THOT MAG-B-NOX does exactly that and it does it in precise manner, covering all the pathways involved in NO synthesis and utilization.

Boosting nitric oxide (NO) Synthesis and Activity

However, the first step in boosting NO synthesis is getting rid of the products, conditions and habits that limit NO synthesis.

And this is where oral hygiene comes in. A large part of nitric oxide production depends on beneficial bacteria living in the mouth. These bacteria convert dietary nitrates (from vegetables or supplements) into nitrites, which are later transformed into nitric oxide in the body^[30].

If these bacteria are damaged or destroyed, NO production drops significantly, no matter how healthy your diet is.

So, everything that imbalances the microbiome in the oral cavity needs to be removed a soon as possible: sugar or sugary products, mouthwash, toothpaste or fluoride containing products, tongue scraping. Using antibacterial mouthwash may feel “clean,” but it kills the very bacteria needed to make nitric oxide. Regular mouthwash use has been shown to reduce NO availability and increase blood pressure^{^[31]}.

Also, it is important to choose nose breathing over mouth breathing – this protects the microbiome in the mouth. Breathing through the mouth dries the oral environment and creates conditions that damage beneficial bacteria. Nose breathing helps maintain a healthier oral microbiome and supports nitric oxide availability^{^[32]}. PS: Nasal breathing itself increases NO levels because nitric oxide is produced in the nasal sinuses and inhaled into the lungs.

Dental infections, gum disease, and untreated cavities also increase inflammation and reduce nitric oxide signaling, further disrupting circulation and healing^[33]. and to fix teeth problems and cavities/infections.

So, even if we have the most powerful tools that we can use to enhance our NO synthesis, let’s not ignore the basics and let’s limit losses. It is not only equally important, it is essential. Always make sure you have the basics in check before, during and after using more advanced instruments.

Even the most advanced nitric oxide boosters cannot compensate for daily habits that block NO production. Protecting the oral microbiome, choosing gentle oral hygiene, breathing through the nose, and fixing dental problems are not optional details — they are the foundation.

Now… once the foundation is covered… Let’s move to “the real deal”.

Let’s see how THOT MAG-B-NOX helps improving NO synthesis through each one if it’s ingredients.

THOT MAG-B-NOX: The energy makeover booster

SHOP MAG-B-NOX

THOT MAG-B-NOX – the most complex supplement formula for boosting NO synthesis

THOT MAG-B-NOX is a very smart supplement that reunites no less than 23 ingredients, all of them very carefully selected to cover all of the mechanisms involved in NO synthesis, preservation and usage.

Now that you know what NO is, what it does and how it works, let’s see how each special ingredient is involved in this.

1. Amino Acids: The Foundation!

Nitric oxide cannot be produced without the right raw materials. And this is the part where almost every genius discovery and advanced technology fails. You can stimulate your body to produce certain things all you want, but, if you fail to provide the raw materials besides the instructions, the body will be forced to take them from other organs and systems and, sooner or later, it will collapse.

So yes, you are the boss, you are the one in charge, you are the one giving the command. But you can lead your body with respect and care, or you can be a tyrant – your choice. Both ways work. But only one of them is sustainable.

This is where Thot Nutrition makes the difference. This is where Thot Nutrition brings a point of difference to the table. This is where Thot Nutrition stands out.

Our supplements understand the big picture. We see the gaps and we fill them. We detect the blind spots and we cover them. Because it is not only about speed and efficiency, but also about long-lasting results and integrity.

The long-term gain doesn’t happen when you sacrifice one organ or system to improve another. The long-term gain happens when you nurture every aspect of your body and move as a whole.

And so, we developed a product that delivers not only the raw materials to generate more Nitric Oxide, but also the raw materials for the body to handle the effects of NO in the body. For example: Yes, NO, once synthesized, stimulates vasodilation, wound healing, stem cell activation and DNA repair… but do you have enough raw materials to support these processes?

This is where amino acids come in. Some are needed for the basic’s functions of the body, some are needed for NO synthesis, and some are needed for carrying the effects of NO.

And now, we will show you how each one in this formula works:

● L-Arginine & L-Citrulline

These two amino acids work hand in hand as primary substrates for nitric oxide synthesis. L-Arginine is the primary substrate for nitric oxide (NO) production by endothelial nitric oxide synthase (eNOS)^{^[34]}. However, oral L-Arginine alone is extensively metabolized during first-pass (intestinal and hepatic arginase activity) before it reaches systemic circulation, limiting its effectiveness as a substrate for NO synthesis^[35].

That’s why THOT MAG-B-NOX also includes L-Citrulline, which is more bioavailable and converts into L-Arginine inside the kidneys, where it avoids first-pass metabolism^[36]. Together, they ensure a sustained production^[37].

● L-Arginine & BPC157 Peptide (L-Arginine is the precursor for BPC157)

Also, Arginine is boosting the pathway for BPC 157 Peptide that works synergic. This miracle small peptide is involved in so many processes:

Collagen Synthesis

BPC-157 increases collagen production and fibroblast activity. This directly affects how strong and well-structured repaired tissue becomes, not just how fast it heals.

Nitric Oxide Modulation

BPC-157 influences nitric oxide, which controls blood vessel dilation and vascular function. Better circulation control means more efficient delivery of repair signals to the injury site.

ERK1/2 Pathway Activation – pathway as a primary mechanism for driving cell growth, regeneration, and angiogenesis in musculoskeletal tissues

BPC-157 activates specific pathways that literally drive cell growth and regeneration. This increases the rate at which damaged cells are replaced with new functional tissue.

Inflammatory Suppression

BPC-157 reduces excessive inflammatory signaling after injury. Less prolonged inflammation means less secondary tissue damage and faster progression into actual repair.

Growth Hormone Receptor Upregulation

BPC-157 increases the sensitivity of tissue to growth-related signals. This may amplify the body’s own repair processes rather than acting as a direct growth driver.

Microvascular Integrity & Blood Flow

BPC-157 improves small blood vessel stability and restores flow in damaged areas. This helps prevent tissue breakdown caused by poor circulation during recovery.

Dopaminergic & Pain Modulation Effects

BPC-157 influences dopamine-linked pathways and reduces early-stage pain signals. This appears to blunt acute pain response, but not long-term or chronic pain.

BPC-157 appears to act as a multi-pathway regulator of the healing, simultaneously influencing blood flow, inflammation, and tissue reconstruction rather than targeting a single mechanism. This coordination may explain its consistent effects in preclinical models, but without robust human data, its true impact on complex human recovery systems remains unresolved.

● L-Lysine

More than just a structural protein component, L-Lysine plays a vital role in collagen synthesis and maintenance – which supports vascular elasticity, blood vessel repair, skin health and beauty and the immune system. (Read Thot Nutrition’s Article on Lysine)

● L-Leucine

L-Leucine is best known for activating muscle protein synthesis via the mTOR pathway^[38]. Indirectly, leucine supports nitric oxide–dependent physiology by improving insulin sensitivity, glucose disposal, and skeletal-muscle nutrient uptake. These metabolic effects enhance endothelial function and microvascular perfusion, creating a physiological environment in which NO-mediated blood flow more effectively supports muscle hypertrophy, tissue repair, and exercise resilience. (Read Thot Nutrition’s Article on Leucine)

● L-Tryptophan

By supporting serotonin and melatonin synthesis, tryptophan helps regulate the nervous system and circadian rhythm – two overlooked but essential modulators of endothelial flexibility and nitric oxide-dependent vascular tone. Melatonin (from tryptophan) directly supports endothelial function and NO signaling^[39]. Serotonin modulates vasoconstriction and vasodilation depending on receptor subtype and endothelial integrity, showing how mood and nervous-system signaling influence vascular tone and endothelial responsiveness^[40]. (Read Thot Nutrition’s Article on Tryptophan)

● L-Methionine

Methionine is a key methyl donor and a central regulator of cellular detoxification, redox balance, and tissue renewal. Through its role in the methionine–homocysteine cycle, methionine supports glutathione synthesis, DNA repair, and liver detoxification pathways. By limiting oxidative stress and preserving endothelial integrity, methionine indirectly supports optimal nitric oxide (NO) bioavailability. In this sense, methionine does not create the NO signal – but protects the biochemical environment that allows NO signaling to function efficiently^[41]. (Read Thot Nutrition’s Article on Methionine)

Together, these 6 amino acids build the architecture for NO synthesis and nourish the systems that sustain it. THOT MAG-B-NOX delivers what your body needs, in the right form, the right ratio, and the right context.

2. Magnesium x5 + Electrolytes: The Bioelectric Support

If amino acids are the building blocks of nitric oxide (NO), magnesium and electrolytes are the systems that allow those signals to work efficiently. Magnesium regulates membrane transport, nerve conduction, muscle contraction (including the heart), vascular tone, and cellular energy production—processes that directly influence endothelial function and NO bioavailability^[42]. Magnesium deficiency is associated with endothelial dysfunction, increased vascular tone, and reduced NO availability^{^[43]}.

The Magnesium Quintet: Five Molecular Keys

THOT MAG-B-NOX is formulated with five distinct forms of magnesium, each selected for its unique tissue-targeting properties and bioavailability profile. These are not generic mineral salts. They are pharmacologically relevant forms that reach the brain, heart, muscles, and cells where real metabolic magic happens.

● Magnesium ascorbate

Magnesium ascorbate combines magnesium with vitamin C, supporting antioxidant defense. Vitamin C helps stabilize tetrahydrobiopterin (BH4), a critical cofactor for endothelial nitric oxide synthase (eNOS), thereby indirectly supporting NO synthesis^[44].

● Magnesium bisglycinate

Chelated to glycine, magnesium bisglycinate is highly bioavailable and gentle on the gut^[45]. It supports neuromuscular relaxation, parasympathetic (calming) tone, and sleep quality. Glycine itself has inhibitory neurotransmitter activity and supports connective tissue and gut integrity.

● Magnesium taurate

Magnesium taurate combines magnesium with taurine, a molecule with strong affinity for cardiovascular and neural tissues. This form supports calcium-channel regulation, membrane stability, and cardiac rhythm, indirectly favoring healthy vascular tone^[46].

● Magnesium Malate

Magnesium malate supports mitochondrial energy production, as malate is a Krebs cycle intermediate^[47]. This form is especially useful in states of fatigue, impaired ATP production, or metabolic stress – conditions that also impair NO signaling.

● Magnesium Citrate

One of the most widely absorbed forms, citrate acts as a gentle laxative, improves bowel motility, and supports electrolyte absorption. In THOT MAG-B-NOX, it balances the system and ensures smooth digestive function – key for consistent nutrient uptake^[48].

Together, these forms span the spectrum of neurovascular, muscular, digestive, and metabolic support—ensuring you’re not just meeting magnesium quotas, but actually absorbing and using it where it matters most.

Electrolyte Intelligence: Potassium, Sodium, and Chloride

In a world dominated by stimulants and diuretics, most people are electrolyte deficient—even if they drink water. Proper hydration is not about volume; it’s about electrical balance. Electrolytes create the gradient that drives muscle contraction, heartbeat rhythm, nerve signaling, and nutrient transport.

THOT MAG-B-NOX includes:

Potassium Citrate – Regulates nerve conduction, blood pressure, and acid–base balance. Essential for muscle recovery, cellular hydration, and vascular tone.
Sodium Chloride – Supports osmotic pressure, brain signaling, and fluid homeostasis. In its purest form, it restores what is lost through sweat and stress.

Along with magnesium, these minerals form the core quartet – Mg, K, Na, and Cl – that stabilizes the internal terrain. They allow NO to be generated, distributed, and utilized with maximum efficiency.

“Electrolyte balance is a cornerstone of cardiovascular health, impacting everything from endothelial function to neurohumoral regulation.” (EFSA Scientific Opinion)

Whether you’re working out, working late, fasting, or recovering, THOT MAG-B-NOX gives your cells what they need to maintain rhythm, flow, and charge.

3. Antioxidants + Polyphenols: Nitric Oxide (NO) Protection & Vascular health

Once NO is generated, it becomes extremely vulnerable to oxidative degradation – especially in inflamed, stressed, or toxic internal environments. That’s why simply producing NO is not enough. To experience the long-term benefits of nitric oxide – mental clarity, physical endurance, sexual vitality, cardiovascular resilience – you need to protect and stabilize it.

This is where THOT MAG-B-NOX shows its multidimensional intelligence. The formula integrates a carefully curated blend of antioxidant-rich plant extracts, polyphenol complexes, and bioavailable vitamin C that together create a biochemical shield for NO.

The ingredients in this category were chosen mainly for their electron donor capacity.

Electron donors play a crucial role in the mitochondrial respiratory chain, enhancing energy production and reducing oxidative stress. This indirectly supports the body’s ability to produce and utilize nitric oxide effectively^[49].

● Red spinach Extract (Oxystorm®)

Oxystorm® is the star ingredient of this formula.

Oxystorm® is a standardized extract of red spinach (plants of the genus Amaranthus – Amaranthus hypochondriacus) – not the common green spinach^[50].

What is special about this extract is its high nitrate content – standardized to ~ 9% nitrate^[51] (i.e. about 90 mg nitrate per gram of powder), which makes its nitrate concentration several times higher than typical nitrate-rich foods like beetroot powder or beet juice!

Oxystorm® is produced via a water-based extraction process, is 100% water-soluble, sugar-free, and reportedly free of oxalates (compounds that in high amounts can interfere with mineral absorption or contribute to kidney-stone risk). These characteristics makes it highly bioavailable!

Because of its high nitrate density and standardized content, Oxystorm® is popular in sports nutrition – many supplement formulas use it to reliably deliver nitrate (NO₃⁻) without the variability seen in whole-food sources^[52].

A portion of circulating nitrate is concentrated into saliva, then reduced by oral bacteria to nitrite (NO₂⁻), which upon swallowing becomes part of the systemic nitrite pool. This nitrite serves as a substrate for NO production in tissues – especially under conditions of low oxygen or high demand (e.g. exercise). A significant portion is recycled through the entero-salivary pathway^[53].

Nitrite is reduced to NO in tissues (muscles, endothelium, vascular smooth muscle) via enzymatic and non-enzymatic pathways – independent of nitric-oxide synthase (NOS), which is useful especially when endogenous NO production is compromised. Given the concentration of nitrate from Oxystorm®, these effects may be more pronounced or reliable than from less concentrated sources.

Some of those studies report improved exercise tolerance, better oxygen utilization (VO₂ kinetics), and enhanced endurance and/or performance^[54]. Human studies using standardized red spinach extract comparable to Oxystorm® demonstrate increased plasma nitrate and nitrite levels, associated with improvements in exercise-related outcomes^[55].

● Hawthorn Berries Extract (5:1)

Hawthorn (Crataegus spp.) is one of the most extensively researched cardiovascular botanicals in European phytotherapy. Standardized extracts – particularly concentrated formats such as a 5:1 extract – are rich in oligomeric procyanidins (OPCs) and flavonoids, compounds known to support endothelial health.

Hawthorn extract has vasodilatory and anti-inflammatory effects via enhancing the NO pathway^[56].

It primarily works by stimulating the body’s own endothelial nitric oxide synthase (eNOS), the enzyme responsible for physiological NO production from L-arginine. This positions hawthorn as a complementary, enzyme-driven NO modulator rather than a direct NO donor^[57].

The cardiovascular activity of hawthorn berries is largely attributed to OPCs and flavonoids such as hyperoxide, vitexin, quercetin, and rutin. Experimental studies show that these compounds activate intracellular signaling pathways (notably PI3K/Akt), leading to phosphorylation and activation of eNOS. As a result, endothelial cells increase NO production directly at the vascular wall. In parallel, hawthorn polyphenols reduce oxidative stress by scavenging superoxide radicals, which would otherwise destroy NO by forming peroxynitrite^[58].

Healthy nitric oxide signaling depends on a functional endothelium. Long-term hawthorn supplementation has been shown to improve endothelial function, arterial elasticity, and coronary blood flow. These vascular effects translate clinically into modest reductions in blood pressure and improved exercise tolerance, particularly in individuals with compromised cardiovascular function^[59].

In addition to NO effects, hawthorn extracts have mild inhibitory activity on angiotensin-converting enzyme (ACE), As angiotensin II suppresses eNOS and increases reactive oxygen species, lower angiotensin II levels indirectly favor NO signaling^[60].

A 5:1 hawthorn extract provides a higher and more consistent concentration of OPCs and flavonoids than whole berries or non-standardized powders. This standardization is critical for reliably influencing eNOS activity and endothelial function^[61].

In nitric-oxide–focused formulations, hawthorn works synergistically with amino acid precursors (L-arginine, L-citrulline), mineral cofactors (magnesium), and nitrate sources (such as red spinach extract), offering dual-pathway support for NO production and preservation. Its clinical history and mechanistic depth make hawthorn a strategic ingredient in advanced vascular and longevity formulations^[62].

● Acai fruits extract (10:1)

Açaí (Euterpe oleracea) fruit extracts, which are rich in anthocyanins and polyphenols, support nitric oxide bioavailability primarily through antioxidant protection of NO, upregulation of endothelial nitric oxide synthase (eNOS), and improvements in endothelium-dependent vasodilation. Açaí does not act by supplying nitrate substrates but instead preserves and enhances endogenous endothelial NO signaling^[63].

It contains exceptionally high levels of anthocyanins and other flavonoids, which are the primary bioactive compounds responsible for its vascular and endothelial effects^[64].

Açaí polyphenols reduce oxidative stress by scavenging reactive oxygen species (ROS) and upregulating endogenous antioxidant enzymes (e.g., superoxide dismutase). This limits NO degradation, thereby increasing functional NO bioavailability^[65].

Experimental studies in endothelial cells and animal models show us that açaí extracts increase eNOS expression and phosphorylation, supporting enhanced endothelial NO production^[66].

Chronic supplementation with açaí extracts in hypertensive rodent models prevents endothelial dysfunction, attenuates blood pressure elevation, and increases eNOS expression – demonstrating long-term vascular protection^[67].

Certain açaí fractions downregulate inducible nitric oxide synthase (iNOS) expression in inflammatory models, reducing pathological NO overproduction while preserving physiological endothelial NO signaling^[68].

Aronia fruits extract (10:1)

Dark purple berries (e.g., aronia, açaí, blueberries) are among the richest dietary sources of anthocyanins and phenolic acids. These bioactive compounds have clinically relevant vascular effects that extend beyond general antioxidant activity, primarily by improving endothelial function and preserving nitric oxide (NO) bioavailability^{^[69]}.

Anthocyanins support NO-mediated vasodilation by reducing oxidative stress in the vascular endothelium. This way, the reaction of NO with superoxide to form peroxynitrite is limited. Human randomized controlled trials and meta-analyses show that anthocyanin supplementation significantly improves flow-mediated dilation (FMD), a validated marker of endothelial NO signaling^[70]

In addition to stabilizing NO, anthocyanin-rich supplementation has been shown to lower blood pressure and improve arterial compliance, effects that are consistent with enhanced endothelial signaling and reduced oxidative burden^[71].

Recent studies also suggest that anthocyanins contribute to cerebral blood flow and blood–brain barrier integrity, probably through their endothelial-protective and anti-inflammatory effects^[72].

Together, these effects form a triad of vascular protection: stabilization of circulating NO, reduction of oxidative stress markers, and restoration of endothelial tone from the inside out.

Non-Acidic Vitamin C (from Magnesium ascorbate)

Vitamin C is far more than a general antioxidant. In human physiology, it functions as an essential metabolic cofactor for collagen synthesis, a process critical for maintaining the structural integrity of blood vessels, capillaries, and connective tissue^[73]. Adequate collagen turnover ensures that vessels remain elastic and resilient under pressure, directly supporting healthy circulation. Deficiency or suboptimal intake weakens vascular walls, increases permeability, and accelerates endothelial dysfunction – conditions that compromise blood flow and nitric oxide (NO) signaling^[74].

Vitamin C plays a direct and well-documented role in supporting endothelial nitric oxide synthase (eNOS) function. It enhances NO bioavailability by maintaining tetrahydrobiopterin (BH₄), a critical eNOS cofactor. When BH₄ is depleted, eNOS becomes “uncoupled” and produces superoxide instead of NO^[75]. Vitamin C helps prevent this uncoupling and shifts endothelial signaling back toward NO production rather than oxidative stress.

One of vitamin C’s most powerful functions is its role in regenerating other antioxidants, particularly vitamin E and glutathione. By restoring these molecules to their reduced (active) forms, vitamin C creates a networked antioxidant system rather than acting in isolation^[76]. This is especially relevant for NO biology, as oxidative stress rapidly degrades NO into peroxynitrite. By lowering reactive oxygen species and reinforcing endogenous antioxidant defenses, vitamin C extends the functional lifespan of NO in circulation.

Delivering vitamin C as magnesium ascorbate has two advantages: improved gastrointestinal tolerance and synergistic mineral support^[77]. Magnesium ascorbate is gentle on the stomach, making higher doses – such as the 800 mg used in THOT MAG-B-NOX – practical for daily use. Magnesium itself supports endothelial relaxation, ATP production, and ionic balance, further enhancing vascular responsiveness. Together, they allow NO to persist longer, tissues to recover faster, inflammation to resolve more efficiently, and oxygen delivery to remain unobstructed.

THOT MAG-B-NOX is not only about optimizing blood flow and performance – it’s also about long-term biological integrity. That’s why it integrates a regenerative layer, addressing the cellular renewal and energy restoration systems that form the backbone of healthy aging.

Moringa Extract (10:1 Concentration)

Often referred to as “The Miracle Tree,” Moringa is one of nature’s most nutritionally complete plants. The extract used in THOT MAG-B-NOX is 10:1 concentrated, meaning that each dose delivers the equivalent of 3000 mg of raw Moringa leaf – rich in:

Quercetin, a flavonoid that lowers inflammation and enhances endothelial function
Chlorogenic acid, which improves insulin sensitivity and modulates blood pressure
Sulfur-containing compounds that boost detoxification (phase II liver enzymes)
Essential amino acids, minerals, and antioxidants that protect mitochondria

Moringa doesn’t act in isolation. It supports the internal terrain where NO can thrive. It ensures that detoxification pathways work, inflammation is managed, and energy substrates are available. Moringa oleifera has antioxidant, anti-inflammatory and cardiovascular protective effects^[78].

4. The B-Vitamin Triad (B3, B6, B12): Powering Mitochondria, Methylation, and Nitric Oxide Signaling

Vitamins B3, B6, and B12 form a functional triad that supports cellular respiration, methylation, and neurological stability. At the cellular level, these vitamins are indispensable for mitochondrial efficiency, redox balance, and amino-acid metabolism^[79]. Because nitric oxide (NO) production and signaling are tightly linked to mitochondrial health and oxidative status, adequate availability of this B-vitamin triad directly influences endothelial performance, energy resilience, and long-term metabolic integrity.

Vitamin B3 (Niacinamide): Fueling NAD⁺, Energy Metabolism, and Vascular Flexibility

Vitamin B3, provided as niacinamide, is a direct precursor to NAD⁺ and NADP⁺, coenzymes essential for mitochondrial respiration, ATP generation, and DNA repair. NAD⁺ availability governs how efficiently mitochondria convert nutrients into usable energy and how well cells respond to oxidative stress^[80].

In vascular tissue, adequate NAD⁺ supports endothelial repair mechanisms and improve vascular elasticity, indirectly favoring NO bioavailability by maintaining mitochondrial and redox balance.

Niacinamide also plays a role in neurotransmitter synthesis and neural energy metabolism, linking vascular health to cognitive and nervous-system function.

Vitamin B6 (Pyridoxine HCl): Amino Acid Metabolism, Homocysteine Control, and Endothelial Health

Vitamin B6 is a critical coenzyme in amino-acid metabolism and neurotransmitter synthesis. It enables the conversion of amino acids into serotonin, GABA, and dopamine – key regulators of nervous-system tone and stress resilience.

From a vascular perspective, B6 plays an essential role in heme synthesis, supporting red blood cell production and oxygen delivery.

It is also central to homocysteine metabolism; inadequate B6 levels are associated with elevated homocysteine, increased inflammation, and impaired endothelial function. By keeping homocysteine in check, vitamin B6 helps preserve vascular integrity and reduce cardiovascular strain^[81].

Vitamin B12 (Methylcobalamin): Methylation, Neurovascular Repair, and Energy Regulation

Vitamin B12, particularly in its bioactive methylcobalamin form, is essential for DNA synthesis, nerve regeneration, and mitochondrial energy metabolism. It plays a central role in methylation pathways that regulate detoxification, neurotransmitter balance, and hormonal stability^[82].

B12 deficiency disrupts homocysteine metabolism and is strongly associated with endothelial dysfunction, cognitive decline, and impaired vascular repair^[83].

Together, vitamins B3, B6, and B12 rebuild the cellular engines that nitric oxide depends on. By enhancing mitochondrial output, stabilizing redox balance, and optimizing methylation, this triad ensures that NO is not only produced and preserved, but functionally integrated into energy metabolism, vascular tone, and neurological resilience^[84].

This is what makes THOT MAG-B-NOX more than a NO booster.

THOT MAG-B-NOX is everything that happens before, during and after NO synthesis.

THOT MAG-B-NOX: The energy makeover booster

SHOP MAG-B-NOX

Recommendations and usage:

We recommend:

1-2 vials /day

[1] The Nobel Prize in Physiology or Medicine 1998

[2] The Nobel Price 1998 – Nitric oxide as a signalling molecule in the cardiovascular system

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[50] PLT debuts standardized nitrate ingredient from red spinach

[51] PLT Health Solutions – Oxystorm® ingredient profile

[52] PLT Health Solutions Teams Up with DolCas Biotech to Market OXYSTORM Standardized Nitrate

[53] Lundberg, J.O., Weitzberg, E. NO generation from inorganic nitrate and nitrite. Nitric Oxide, 2010.

[54] Oxystorm – Red Spinach [Amaranthus hypochondriacus] leaf 9% Nitrates [HPLC] powder extract

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Thot Leptin RX – The Eloquent Solution to Losing Weight in 2024 (World Premiere Formula)

ThotNutrition2025 — Wed, 10 Apr 2024 18:40:34 +0000

Table of Contents

Synopsis. 2

Introduction. 3

Weight Gain and Obesity. 5

Leptin and Leptin Rx. 5

What is leptin and what does leptin do?. 5

What is leptin resistance (Leptin Rx)?. 6

How do you know you have Leptin resistance (Leptin Rx)?. 7

Our Solution: Thot Leptin Rx. 8

Thot Leptin Rx: The formula. 8

Part I: Leptin Rx optimization. 9

1. Acetyl-L-Carnitine HCL. 10

2. L-Carnosine. 11

3. L-Taurine. 12

4. D-Ribose. 13

5. L-glutamic acid. 14

6. L-Cysteine. 15

7. L-Lysine monohydrochloride. 15

Part II: Burning booster and Metabolic enhancers – “the magic 4 combo”. 16

1. Ginger root extract 18

2. Black pepper extract 19

3. Cayenne pepper extract 21

4. Garlic extract 22

1. Green coffee bean extract 23

2. Garcinia cambogia extract 24

Part III: Energy support (antioxidant, anti-inflammatory and hypocholesterolemic agents) 25

1. Glycine. 25

2. L-Glutamine. 26

3. Ascorbic acid. 27

4. Black currant extract 28

5. Zinc oxide. 28

Benefits of Thot Leptin Rx. 29

Usage and Dosage. 29

Conclusion. 30

Synopsis to burn fat in 2024

We are offering an elegant and very advanced solution to losing weight. A world premiere formula that reactivates the natural burning cycle of the body and optimize brain to cell communication, acting as a specific nootropic.

Formulated by the nutritionist and biohacker Bralgei Shackry – aka – Gabriel Pesa

I offer this magic formula in an open source format. I believe that the world deserves this. The only condition that I require is this: Mention the creator/formulator (Bralgei Shackry aka Gabriel Pesa) and the source of information (Thot Nutrition).:.

Leptin Rx – The solution for Leptin resistance and fat burning mechanisms inhibition.

Thot Leptin RX by Gabriel Pesa aka Bralgei Shackry is licensed under Creative Commons Attribution-ShareAlike 4.0 International

PRODUCT NOTIFICATION

Notification certificate for Thot Leptin RX: National Institute for Public Health – Seria AC 4466/Nr: 1063/03.04.2024

The science may be overwhelming, but we will try to make it simple for you, since we have now one pill that does a multitude of tasks for the same end result – teach your body again how to burn fat and get healthy.

We have been looking in the wrong direction. Weight loss is a communication problem, and, as a species, we are faced with the same lesson at the cellular level.

It is one thing to have cell phones and available network for sharing info, and it is another thing to really communicate.

We do not know how to really communicate. Same lesson; different level. So let’s crack this open and solve it once and for good.

Leptin Rx – The solution for Leptin resistance and fat burning mechanisms inhibition.

World Premiere Formula

LEPTIN Rx

The first natural formula that helps you reclaim your metabolic burning rate and reclame your magic back.

18 natural ingredients – one pill.

SHOP

Thot Leptin Rx represents a breakthrough in the realm of weight management. Based on more than 30 years of experience in nutrition, biohacking and antiaging, and a meticulously crafted blend of 18 natural active ingredients, this dietary supplement represents a paradigm shift in the area of weight loss and brain optimization.

Unlike conventional approaches that focus solely on removing the excess fat or modifying the mechanisms that lead to fat accumulation, Thot Leptin Rx targets the true root cause: miscommunication within the body – between cells and the brain. In other words: the problem is not you, nor your diet or the fat on your hips and belly. The problem is not the way your cells or your organs work – all of these are the consequence of the faulty communication within your body!

As we all know now for sure: It’s not the sugar that is wrong, it’s the excess of it. The excess sugar is not burned. Instead, it is converted to fat, because the burning cycle is corrupted.
bralgei shackry

Through a multifaceted strategy, Thot Leptin Rx restores the energy balance in your body and helps you burn fat through three main mechanisms:

Firstly, it targets leptin resistance – which often makes it hard to lose weight, by helping the brain and the body become more sensitive to leptin, also known as “the satiety hormone”.
Secondly, it boosts the body’s fat-burning processes, making metabolism work better and faster. The more energy you have, the more fat you burn.
Thirdly, and most importantly, it restores and improves the energy for the communication pathways between the brain and the body, and between all cells, which promotes overall well-being.

The uniqueness of Thot Leptin Rx lies in taking the conventional weight management approaches to the next level of understanding. Rather than viewing weight gain as a cellular or organ-centric issue, the attention moves on the intercellular and brain-body communication obstacles.

By shifting the focus to this mechanism, Thot Leptin Rx changes the current weight loss paradigm and opens a door to new possibilities in weight loss research and practice and encourages new pathways to be explored and used to obtain and prolong health and wellness, in a holistic and integrative way.

Thot Leptin RX is not a magic pill, but it gives your magic back.
bralgei shackry

Leptin Rx – The solution for Leptin resistance and fat burning mechanisms inhibition.

Introduction to losing weight smart

Weight gain and obesity is a very complex situation to handle and has more than a few mechanisms involved. If weight management was easy to solve, this article wouldn’t have been written and this supplement wouldn’t have been created.

You cannot fool your cells with empty food for too long. In truth, many of fat and obese people are in fact starved at cellular level. It is a paradox and the medical community needs to wake up to reality.

Cells are undernourished. They are screaming for real food, and, on top of it, the “phone line” to the brain is dead and nobody listens (Leptin Rx) – if you understand my metaphor.

To have a deeper and larger understanding about hunger, please watch the following videos, recorded in Romanian language: Nimic nu bate foamea – Partea 1 (Nothing beats hunger – Part 1) and Nimic nu bate foamea – Partea a 2-a (Nothing beats hunger – Part 2).

Hunger is mostly acknowledged on the physical plane. But, make no mistake; if you have it on the physical, you have it on the emotional, mental and spiritual as well. So ask yourself: what is it that really feeds you?

For the ones who dare to work harder, to discover and to experience more, I invite you to join my exclusive Master Training Classes.

Basically, in order to transcend this hunger, first, you have to accept the hunger. Until you accept hunger, you will fight it. The question is: how much longer are you willing to fight with yourself? This is how leptin resistance feels like. And this acceptance involves two important elements:

you need to feel what you and your body really need
you need to be honest about what it is that you feel

You can only feel with your body. There isn’t any other instrument outside of your body. The body doesn’t lie. But, if your body is numbed and silenced for too long, can you trust it then? This is why it is so important, first and foremost, to restore the receptors and the communication within the body. Now you can feel it and trust it. Now you can feel and trust yourself again. Now you can choose for real.

Humanity has been living in hunger for too long. It is time to get out of there. It is time to thrive. It is time to love and to feel loved. But then again, first, you have to feel. Everyone knows hunger, fear and cold. It is time to get used to abundance, love and warmth. And each and every one of you can do it. Do it one step at a time, by starting with your cells.

It is possible and now we have all what we need. Do not push yourself more than necessary. But be honest with yourself, and that which you can do, do it! Every little gesture matters.

Changes on the physical plane will automatically reflect on more subtle planes.

Ideal weight is hard to maintain over time, due to lack of proper nutrients and waste accumulation, which, gradually, deteriorate the metabolic functions in the whole body. Therefore, in order to obtain a long-lasting result in losing weight, every one of these mechanisms should be approached and unlocked.

Most of the people trying to lose weight cover a few of these mechanisms and very little to none cover them all. This is what Thot Nutrition is all about. Every ingredient is carefully chosen for its precise role in regulating all of the mechanisms involved in weight management.

Leptin Rx – The solution for Leptin resistance and fat burning mechanisms inhibition.

Thot Leptin Rx is the only supplement on the market that covers all the mechanisms involved in weight management in just one pill.
bralgei shackry

One of the most important and overlooked mechanisms when it comes to losing weight is leptin resistance[1], also known in the medical world as „Leptin Rx”. This is where the name Thot Leptin Rx comes from. The full mechanism will be described in detail later in this article.

Thot Leptin Rx has a unique formula, based on more than 30 years of experience in nutrition, biohacking and antiaging, and contains no less than 18 active ingredients. This one-of-a-kind formula is designed to support weight loss and overcome leptin resistance.

The purpose of this article is to provide an in-depth overview of Thot Leptin Rx and its potential benefits for individuals struggling with overweight and obesity, a consequence of the metabolic disorders. The article will provide both the big picture and the details regarding this problem.

So… Let’s dive-in!

Weight Gain and Obesity

Obesity has become a global epidemic, with its prevalence steadily increasing over the past few decades. According to the World Health Organization (WHO), obesity has nearly tripled worldwide since 1975, with more than 1.9 billion adults considered overweight, and of these, over 650 million are obese. This alarming trend is not limited to adults, as childhood obesity rates have also risen significantly in recent years.

Obesity is a condition arising from a persistent mismatch between energy intake and expenditure, leading to a nutritional imbalance. This disorder is marked by inflammation affecting various cell types.

This condition increases the risk of serious health issueslike diabetes, heart disease, and cancer. Leptin resistance, a condition where the body becomes less responsive to the hormone leptin, is a major contributor to obesity.

Leptin and Leptin Rx

What is leptin and what does leptin do?

Leptin is a hormone which regulates appetite and metabolism. Leptin is produced mainly by fat cells and plays a crucial role in regulating appetite, metabolism, and energy balance.

When functioning correctly, leptin signals to the brain that the body has had enough to eat, that the body has stored enough energy and that it is ok to stop eating. Leptin is “the satiety hormone” and it reduces appetite.

The complementary hormone is called “ghrelin” and it is known as “the hunger hormone”, as it signals to the brain that the body needs to eat, thus stimulating appetite. Ghrelin is a hormone primarily produced by cells in the stomach and pancreas and it typically rises before meals and decreases after eating.

This hormone stimulates the release of growth hormone from the pituitary gland and activates brain regions that control appetite.

Basically, together, leptin and ghrelin maintain energy homeostasis and optimize metabolism in the body.

However, things are not always happening by the book, as is the case of leptin resistance (Leptin Rx) and this is happening when various problems start to occur in the body.

What is leptin resistance (Leptin Rx)?

In individuals with leptin resistance, the brain does not receive the signal from leptin, leading to increased hunger, reduced metabolism, and difficulty losing weight. So, although the body has enough stored energy, the brain is in a permanent state of hunger.

Why is that?

Scientists have studied this mechanism for years now. However, it is one of the mechanisms that needs further studies in order to be completely understood. One of the mechanisms involved is related to the leptin receptors – either they are dirty/defective or their regulation is not done properly. Restoring leptin receptors involves both cleaning the receptors and repairing them.

Leptin resistance often goes hand in hand with insulin resistance, another metabolic condition where the body’s cells become less responsive to insulin, a hormone that regulates blood sugar levels, by allowing the glucose to enter the cell. Insulin resistance can lead to high blood sugar levels, which, over time, can increase the risk of type 2 diabetes and other health problems.

There’s a complex relationship between leptin resistance and insulin resistance. Leptin resistance can contribute to insulin resistance by promoting inflammation and disrupting metabolic processes. On the other hand, insulin resistance can worsen leptin resistance by affecting the signaling pathways involved in leptin function.

Both conditions often coexist and can exacerbate each other, creating a vicious cycle that contributes to weight gain and metabolic dysfunction, but not necessarily. Some scientists say that, generally, leptin resistance precedes insulin resistance with 5-7 years. This means that, if detected early, leptin resistance can be reversed and insulin resistance can, therefore, be prevented. However, they can exist separately as well.

Leptin resistance, along with insulin resistance, can determine adrenal resistance. The body becomes, due to the permanent state of hunger, to be in a permanent state of alert. It is known that, in the alert state, cortisol, the stress hormone, is secreted in the adrenal glands. This permanent secretion of cortisol leads to adrenal exhaustion.

It is important to know, that, even if most of the leptin receptors are in the brain, there are some in other parts of the body as well. This being said, leptin can also modify insulin sensitivity in the muscle and liver. If the muscle is sensitive to leptin, the energy (glucose) is used properly – it enters the muscle and it is transformed in energy molecules (ATP). However, if the muscle is resistant to leptin, the energy is stored outside the muscle: either in the adipose tissue as fat or back in the liver as glycogen – and this is another path through which leptin resistance contributes to weight gain.

How do you know you have Leptin resistance (Leptin Rx)?

The diagnosis[2] of leptin resistance can be made clinically or by dosing the hormone “reverse T3” in the blood, but I will deepen this aspect a little later. Clinically, the symptoms that may occur are: obesity, increased appetite, craving for carbohydrates, chronic fatigue.

It’s just the body’s need to get proper food and nutrients. The body needs to be nourished and you cannot fool the cells for too long.

The lack of the right nutrients corrupts the body and triggers a “survival mode” in the whole organism. Proper nutrition manages to feed the cells and to pull it out of the state of siege.

To discover more about Leptin Rx and its mechanisms involved in metabolic disorders, we encourage you to read an article Ketogenic nutrition in metabolic imbalances. The article describes in detail what is leptin resistance and how all metabolic imbalances are related to this mechanism. It covers medical terms, the mechanism of action, the links between leptin and other hormones, organs and systems and, last but not least, it offers a basic approach, starting with a well-done ketogenic nutrition.

Ok, now you know you have it. The question is: what cand you do about it?

We can reverse it step by step, in a way similar to reverse engineering, due to the fact that we still don’t understand many of the complex mechanisms that drives the cells to adapt to hunger and to prolonged nutrient deprivation.

In the next section, we’ll explore how the new product Leptin Rx from Thot Nutrition targets leptin resistance, fat burning and effective communication in the body and how it supports weight loss.

We’ll discuss its unique formulation, how it works, and its potential benefits for people struggling with weight due to leptin and/or insulin resistance, low metabolism rate and miscommunication between the brain and the body and between all the cells.

In addition, we will offer other nutritional instruments to help you achieve your goal.

Our Solution: Thot Leptin Rx

Thot Leptin Rx is specifically formulated to address leptin resistance and promote effective weight loss, utilizing a combination of 18 active ingredients with scientifically proven efficacy in fat accumulation (the reason is more complex than we can comprehend now; we are just in the early stage of understanding these processes).

Each ingredient in Thot Leptin Rx contributes to its overall efficacy in supporting weight loss and improving leptin sensitivity, including enhancing metabolism, suppressing appetite, and promoting fat burning.

It is important to understand that, administered separately, each ingredient contributes in weight management to a certain level. When combined, your metabolism is supported by least 18 different natural ingredients, which activate a lot more pathways that this humble article contains.

Together, the 18 ingredients target different aspects of leptin resistance and metabolic.

Thot Leptin Rx is the most complex and smart formula on the market dedicated to weight management at this very moment. However, do not neglect your diet, sleep and sport habits in order to get the best results. If you want more practical solutions:

Check here the Master AMPK/mTOR Protocol to optimize your fat burning process.

Thot Leptin Rx: The formula

Thot Leptin RX by Gabriel Pesa aka Bralgei Shackry is licensed under Creative Commons Attribution-ShareAlike 4.0 International

Thot Leptin Rx has a unique formula, based on more than three decades of experience in nutrition, and it reunites 18 active ingredients: amino acids, minerals, special carbohydrates and a very effective mix of plants and spices – some of them already famous when we talk about weight management.

Nothing in this formula is random and every ingredient is chosen for its specific effect in the body. The ingredients are perfectly blended not only to work together, but also to potentiate one another, in order to fulfill their purpose and serve the 3 main mechanisms:

We will take every ingredient one at a time and reveal the mechanisms they touch, providing scientific evidence to support the benefits of Thot Leptin Rx.

So let’s see the first part of the formula. This part is made of ingredients, mainly amino acids, which are serving one purpose – restoring and improving brain activity and communication between the brain and the rest of the cells in the whole body, optimizing leptin resistance.

Leptin Rx – The solution for Leptin resistance and fat burning mechanisms inhibition.

Part I: Leptin Rx optimization

Acetyl-L-Carnitine HCL
L- Carnosine
L-Taurine
D-Ribose
L-Glutamic acid
L-Cysteine HCL
L-Lysine HCL

Part II: Burning booster and Metabolic enhancers – “the magic 4 combo”:

Ginger root powder Zingiber Officinale
Black pepper extract 95% HPLC (Biopiperin (1-piperoylpiperidine) )
Cayene Peper (Capsicum Annuum) 5:1
Garlic Extract (Allium Sativum) 1:100

Co-boosters:

Green Coffee extract
Garcinia Cambogia Extract (HCA 60%)

Part III: Energy support (antioxidant, anti-inflammatory and hypocholesterolemic agents)

Glycine (hydrochloric glycine)
L-Glutamine HCL
Ascorbic Acid (vitamin C) 100%
Black currant extract (Ribes nigrum) (Anthocyanidines ≥25%)
Zinc (Zinc oxide)

We will take every ingredient one at a time and reveal the mechanisms they touch, providing scientific evidence to support the benefits of Thot Leptin Rx.

So let’s see the first part of the formula.

Part I: Leptin Rx optimization

Made of: Acetyl-L-Carnitine HCL, L- Carnosine, L-Taurine, D-Ribose, L-Glutamic acid, L-Cysteine HCL, L-Lysine HCL. This part contains 7 ingredients, mainly amino acids, which are serving one purpose – restoring and improving brain activity and communication between the brain and the rest of the cells in the whole body, optimizing leptin resistance.

Of course they have other functions, but the main target restoring leptin resistance.

Some studies have already showed that certain nutrients found in whole foods, like taurine, creatine, carnosine, anserine, and 4-hydroxyproline, are good for preventing and treating obesity, heart problems, aging-related issues, and even cancer, while boosting overall health in people of all ages[3]. Some of them are found in our formula: taurine, creatine, carnosine.

These nutrients are very rarely found in plants and easy to find in red meat, including beef, and nature still teaches us that most nutrients work better when combined with other specific nutrients. As nutrition has been so “corrupted” in the recent years, with vegan and vegetarian diets in trend, the human body might have severe nutrient deficiencies.

Therefore, in order to support optimal growth, development, and overall health, these nutrients have to obtained through diet or supplementation.

Let’s see every one of them separately.

1. Acetyl-L-Carnitine HCL

Acetyl-L-Carnitine (also known as ALCAR) is a version of L-Carnitine with an acetyl group added. L-Carnitine is very popular, especially at the gyms, for its fat-burning effects. But, compared to L-Carnitine, Acetyl-L-Carnitine is more bioavailable than L-Carnitine and it easily crosses the blood-brain barrier (BBB). That is the key!

What does this mean? It means that Acetyl-L-Carnitine is more easily absorbed in the bloodstream and it has all the benefits that L-Carnitine has, but this doesn’t happen the other way around. Moreover, by crossing the BBB, Acetyl-L-Carnitine has some very useful effects in the brain, which the other one cannot, simply because it cannot get in the brain.

Acetyl-L-Carnitine (ALCAR) is known as a nootropic in the biohacking world. Acetyl-L-Carnitine serves as a precursor to acetylcholine, a neurotransmitter that enhances memory, boosts perception, and improves mental clarity.

Now, why is this present in out supplement? Because restoring brain activity to its optimal parameters allows the brain to lead the body’s mechanisms correctly, including the mechanisms involved in weight loss.

The brain itself needs to be awakened and conscious of the faulty communication between the body and the brain.

You all need to understand a basic fact: the cell does not feed with food, but with ATP. All metabolic processes are just a huge effort to transform the food into pyruvate or ketones in order to produce ATP for the cells.

Acetyl-L-carnitine helps in transporting fatty acids (or simply – fats) to the mitochondria. In the mitochondria, they are transformed in energy molecules (ATP molecules). This gives the brain and the body the necessary energy to work properly. As a result, the brain has energy to work well and the body’s metabolic functions are improved: this means fat burning is getting better.

Acetyl-L-Carnitine is naturally synthesized in the body, making it generally well-tolerated and safe for consumption.

Both L-Carnitine and Acetyl-L-Carnitine show potential in improving mitochondrial function and reducing oxidative stress[4]. This effect in the hypothalamus, the part of the brain responsible for regulating appetite and metabolism, may help improve leptin sensitivity.

Acetyl-L-Carnitine modulates the release of aminergic neurotransmitters and the biosynthesis and release of glutamate[5]. Glutamate is the most abundant excitatory neurotransmitter in the central nervous system of adult mammals and has a major role in neuroplasticity. By being a glutamate modulator, ALCAR has the potential to reduce appetite, contributing to weight loss.

Acetyl-L-Carnitine is naturally synthesized in the body, making it generally well-tolerated and safe for consumption.

2. L-Carnosine

L-Carnosine, or β-alanyl-L-histidine, is known as the “longevity molecule”. It is an amino acid made up of histidine and beta-alanine, naturally present in the body, with its highest concentrations in organs which require high amounts of energy: brain, heart, and muscles.

L-Carnosine is an antioxidant. Weight gain, leptin and insulin resistance, diabetes and obesity are already known to be related to mitochondrial dysfunction and oxidative stress. It’s a heavy-metal scavenger and a very efficient toxin remover.

L-Carnosine is also an anti-glycating agent. Sugar-mediated protein crosslinking is a chemical process that occurs when sugar molecules react with proteins, leading to the formation of AGEs – these are harmful molecules which can accumulate in tissues over time, particularly in individuals with poorly controlled blood sugar levels, such as those with diabetes [6] – this makes L-Carnosine a potential therapeutic option for metabolic imbalances like diabetes.

L-Carnosine has also been described as an anti-aging peptide. It was found to suppresses cell senescence in cultured human fibroblasts.

L-Carnosine, commonly used in exercise physiology to boost performance, shows potential in helping prevent and treat conditions like obesity, insulin resistance, type 2 diabetes, and complications related to diabetes, such as cardiovascular disease and stroke [7].

3. L-Taurine

L-Taurine is considered a “conditional” amino acid because the body can produce some of it naturally. The rest of the taurine the human body needs is found in seafood, meat, poultry and eggs. Taurine is synthesized from amino acids methionine and cysteine.

In the firs step of the leptine resistance, monosodium glutamate is starting to inhibit natural production of the taurine. So we put it back on track.

In the body’s fat tissue, taurine is made actively. Its production raises significantly as preadipocytes mature into fat cells. Healthy lean fat cells produce and release a lot of taurine. In hypertrophic obese fat cells, this production slows down, leading to a low level of taurine, associated with obesity.

The cause is not in the cells; the cause is the miscommunication.

The liver is tricked into perceiving that L-Taurine is needed in lower quantities than the body actually requires in order to function properly. This happens because of some inhibitory mediators in the food. MSG is the first culprit.

The anti-obesity effects of taurine are partially attributed to its ability to reduce inflammation in fat tissue [8].

An international epidemiological study suggested that taurine might help alleviate metabolic disorders like obesity, hyperlipidemia and hypertension. Taurine’s efficiency comes from its actions in both peripheral tissues and the central nervous system (CNS).

Taurine helps in obesity primarily through these mechanisms:

increases energy usage by improving fatty acid oxidation (fat-burning)
- decreases lipogenesis (fat production) by reducing glucose oxidation after meals
- regulates the energy usage for glycogen synthesis vs lipogenesis
- suppresses and inhibits oxidative stress
- reduces fat cells inflammation
- supports mitochondrial health

It is possible that low taurine levels can worsen adipocyte dysfunction, which lead to even lower taurine levels, causing a vicious cycle of obesity [9].

Taurine also facilitates the conversion of cholesterol to bile acids and helps eliminate them, lowering the cholesterol levels [10].

4. D-Ribose

D-ribose is a naturally occurring sugar molecule that plays a key role in the production of ATP (adenosine triphosphate), the primary energy currency of cells [11]. One mechanism by which D-ribose may impact obesity is through its role in energy production. This way, D-ribose supplementation could enhance cellular energy metabolism and promote fat oxidation, contributing to weight loss.

In other words, the more energy you have, the more fat you can burn!

Additionally, D-ribose has been studied for its potential benefits in improving exercise performance [12], which could indirectly impact weight management by promoting physical activity.

Furthermore, D-ribose has been investigated for its potential role as an antioxidant [13], which is associated with obesity and its related complications. By reducing oxidative stress, D-ribose supplementation may have beneficial effects on overall metabolic health.

5. L-glutamic acid

L-glutamic acid is a neurotransmitter, transmitting signals between nerves or neurons, in the brain. The active form, glutamate, is involved in cognitive functions such as learning and memory.

There are some terms like glutamate and monosodium glutamate (MSG) which are often misunderstood and misused. So, let’s clarify on them[14]. There are two main forms of glutamic acid: free glutamic acid (D-glutamic acid, not found in nature) and bound glutamic acid (L-glutamic acid, found in nature, bound to protein). Both forms transform in glutamate, once they are in the body. Glutamate is just the protonated form of glutamic acid. But not both of the glutamate forms are useful for the body.

The form of glutamate coming from L-glutamic acid is the neurotransmitter that the human brain needs, while the other form of glutamate is the harmful one, with hyper excitatory effects, in free form – like MSG. Excessive glutamate activity can lead to overstimulation of neurons by glutamate resulting in cell damage.

In other words: “we are fighting fire with fire”
bralgei shackry

Glutamic acid is known for creating umami, one of the five primary tastes, particularly when it’s utilized as a flavor enhancer in the form of monosodium glutamate (MSG) – which isn’t the case in our product, we use the L-glutamic acid form, which is bound to proteins. Excellent sources of glutamic acid include meats, poultry, fish, eggs, dairy products, and kombu. Certain protein-rich plant foods also contain glutamic acid. Glutamic acid makes up approximately 30% to 35% of gluten.

As a neurotransmitter, glutamic acid is an excitatory amino acid neurotransmitter, meaning it enhances the activity of neurons and encourages the transmission of nerve impulses.

In the brain, glutamic acid acts on specialized receptors known as “glutamate receptors”, particularly the NMDA and AMPA receptors. Glutamic acid has an agonist effect on the release of dopamine in the brain.

The brain needs a balance between excitation and inhibition[15]. Glutamate is the main excitatory and GABA the main inhibitory neurotransmitter in the brain, which helps reduce neuronal excitability and promotes relaxation. Glutamate is the metabolic precursor of GABA, which can be recycled to synthesize glutamate. So, if working well, the brain has its mechanisms and feedback loops to regulate these complementary neurotransmitters.

Glutamate is a neurotransmitter involved in signaling satiety to the brain, probably due to its capacity to regulate dopamine release. Some studies suggest that glutamate signaling may influence appetite and food intake, potentially impacting weight regulation. Additionally, glutamic acid is involved in the synthesis of glutathione, which we already know it is essential in metabolic health.

6. L-Cysteine

One mechanism by which L-cysteine may impact obesity is through its role in glutathione synthesis, the body’s most powerful antioxidant. Oxidative stress is associated with obesity and its related complications, so increasing glutathione levels through L-cysteine supplementation may help.

Furthermore, L-cysteine is involved in the regulation of insulin sensitivity and glucose metabolism. Some studies suggest that L-cysteine supplementation may improve insulin sensitivity and help regulate blood sugar levels, which could indirectly impact weight management. There are some contradictions in the literature regarding the effect of L-cysteine in obesity. However, its roles in the body suggest it may be helpful.

Additionally, L-cysteine is a precursor to taurine, another amino acid that has been studied and added to Thot Leptin Rx’s formula for its potential role in weight regulation and metabolism.

7. L-Lysine monohydrochloride

L-Lysine is an essential amino acid that cannot be produced by the body and must be obtained through diet or supplementation. To read more about L-Lysine and all about its benefits, please check out our article: L-Lysine the best and only key to collagen and healthy body in 2023.

L-Lysine is the amino acid which is indispensable in the post-translational acetylation of proteins. This means that the final structure and function of proteins depend on L-Lysine. For a protein to function properly, it needs to undergo some transformation after it is translated from the DNA. Acetylation of proteins is one of these transformational processes.

Acetylation is important in both immunological and metabolic pathways, including the synthesis of metabolic enzymes, and may regulate the balance between energy storage and expenditure.

Obesity, type II diabetes and cardiovascular disease (metabolic syndrome) are widely recognized as features of a chronic inflammatory state[16], therefore benefiting from an optimal immune system.

The monohydrochloride form of the L-Lysine we use makes it easier to absorb and to be used by the body. To buy the best L-Lysine available on the market, please visit our product page: Thot L-Lysine HCL – BEST Professional Grade (100% Plant based).

• • •

Oooooook. We’re done with the first part in the formula. Hope you are good so far. As you have seen, most of it is made of amino acids. They have synergic effect regarding fat metabolism. Moreover, they target not only the metabolic pathways that take place in the periphery, but they also regulate the hunger mechanisms directly from the central nervous system. It is already known that if the brain is well, the body will follow!

Moving on to the next category, which is formulated with plants.

Now… the herbs. This is where we use the power of nature at its best. Traditional medical practices have been using the power of plants since ancient times. But, as usual, it’s better when it is supported by science.

For this part of the formula, we have chosen no less than 6 herbs, each one with very powerful active compounds for fat loss and metabolism and pretty famous in the area of weight management. They work very well if administered separately, but, when they are combined optimally, well… this is when the magic happens.

The selection of the herbs, again, is in no way random. Traditions and habits, as well as medical practices, have been studied in detail for years and years. In this research, I have observed that Asian people use a lot of spices. This part is mostly related to the “burning fat” part of the formula.

Part II: Burning booster and Metabolic enhancers – “the magic 4 combo”

Made of the 4 main ingredients: Ginger root powder Zingiber Officinale, Black pepper extract 95% HPLC (Biopiperin (1-piperoylpiperidine) ), Cayene Peper (Capsicum Annuum) 5:1, Garlic Extract (Allium Sativum) 1:100 and the Co-boosters: Green Coffee extract and Garcinia Cambogia Extract (HCA 60%).

You need to understand that in fact, these 4 ingredients are packed with more than meets the eye. They are full of complex chemicals called terpenes or essential oils compounds. There is the magic.

Together with the amino acids and with the other ingredients, they help the body produce, hold and correctly use energy.

Studies have discovered various natural compounds from peppers, ginger, Japanese pepper, garlic, durian, royal jelly, myoga, and galangal that activate some receptors, known as “pain receptors”. These receptors play roles in regulation of body temperature regulation, inflammation, and the perception of taste and smell, showing that. When activated, fat-burning may be accelerated. Cinnamaldehyde, found in cinnamon, also inhibits fat storage. These findings suggest that certain compounds could help prevent obesity, although their effects on humans needs more investigations [17].

Moreover, giving overweight and obese individuals a mix of spices containing black pepper, cinnamon, cloves, garlic, ginger, oregano, paprika, rosemary, and turmeric is shown to lower triglyceride levels and the release of interleukin (IL)-1β (inflammatory molecule) triggered by high-fat meals [18].

As many have noticed, including myself, people who use a lot of spices and natural stimulants like coffee are very slim, have a great vitality and are very … alive. So, I studied… and I studied… Until the perfect blend to finally solve this leptin resistance and weight gaining problem was complete and functional! This formula is a beast!

Not to brag, but this might actually be the first and only natural and complete formula for Leptin Rx that works.

So, let’s see the plant extract involved.

• • •

Each one of the 4 ingredients is known for a specific compound that helps boosting the metabolic functions: ginger is known for zingiberene, black pepper for piperine, cayenne pepper for capsaicin and garlic for allicin.

What is important to understand is that, although these compounds are very powerful on their own, besides the fact that the composition of each plant extract is much more complex, they work together better than you can imagine.

Their activity is supported by the amino acids presented in the first part and enhanced by another two fat-burners: green coffee extract and Garcinia cambogia extract.

1. Ginger root extract

Ginger (Zingiber officinale, Zingiberaceaefamily) is renowned as both a popular spice and a medicinal herb. It contains volatile oils, comprising approximately 1% to 3%, which include terpenoids like zingiberene, as well as potent compounds such as gingerol, shogaol, and zingerone. Among these, gingerols and shogaols are recognized as the primary bioactive elements of ginger.

In traditional medicine, powdered ginger is recommended for several conditions like nausea, digestive issues, muscle and joint discomfort, and cold symptoms. Its diverse pharmacological benefits have been documented, including its anti-nausea, anti-inflammatory, and antioxidant properties, as well as its potential to regulate glucose and lipid levels and exhibit anticancer effects.

Experimental studies have shown promising results in managing obesity, both in terms of prevention and treatment. Ginger may influence obesity through several possible mechanisms, such as enhancing thermogenesis, promoting fat breakdown, reducing fat production, limiting the absorption of fats in the intestine, and regulating appetite.

One study shows that ginger extract can stop weight gain and reduce fat. Also, it lowers fat levels in the blood and liver, helps remove fat through feces, and reduces the activity of genes linked to fat synthesis and inflammation [19]. The research also shows that this extract activates AMPK in fat tissue – this is a mechanism that is activated during fasting or exercise, when the body burns fat and uses the energy from fats to repair the body. We want this mechanism active when we want to lose fat.

For optimizing the AMPK/mTOR pathways in your body please visit the Master AMPK/mTOR Protocol.

These pathways suggests that ginger extract might be useful as a healthy food to fight obesity and inflammation.

Active components of ginger seem to have agonistic effect one of the temperature sensors and cause the activation of sympathetic nerve system. The sympathomimetic effect of ginger has some important effects in adipose tissue such as increasing the thermogenesis and lipolysis. Moreover, the active components of ginger decrease lipogenesis. Ginger can also suppress intestinal absorption of dietary fat by decreasing lipase enzyme[20].

In addition, ginger has modulatory effects on 5‐HT receptors (serotonin). Ginger can decrease the appetite, maybe through binding to 5‐HT2c receptors in the central nervous system. On the other hand, ginger may act as an appetizer through intestinal 5‐HT3 receptors[21]. In other words, ginger may have the ability not only to suppress appetite, but to actually regulate appetite.

2. Black pepper extract

Black pepper, scientifically known as Piper nigrum and belonging to the family Piperaceae, has garnered considerable interest because of its abundant phytochemicals and bioactive properties. Cultivated primarily in tropical areas like Indonesia, Brazil, and India, the appeal of peppercorns lies in their flavorful compounds, known for their spicy and pungent characteristics.

Black pepper is a popular spice used worldwide in a variety of sauces and culinary creations, including meat dishes. Its key component, piperine, is an alkaloid known for its numerous health benefits. Piperine content within black pepper is estimated to range from 2–10%. Plants belonging to this family are known for their abundance of other active compounds like flavonoids, phenolics, amides, alkaloids, steroids, neolignans, lignans, chalcones, terpenes, and various other phytochemicals.

It is known to have a wide range of therapeutical properties, including antioxidant, antitumor, antihypertensive, anti-asthmatic, analgesic, antipyretic, anti-diarrheal, anti-inflammatory, anxiolytic, antispasmodic, hepato-protective, antidepressant, antibacterial, immunomodulatory, antifungal, antiapoptotic, antithyroid.

Moreover, it enhances cognitive functions and fertility.

Studies have shown that the absolute quantities of absorbed piperine were relatively higher compared to similar spices with a close structural makeup, such as curcumin.

Studies found out that black pepper has the following benefits in metabolic disorders [22]:

Decreases blood sugar level
Increases the insulin secretion
Decreases inflammation
Supports a high-fat diet in body weight, body composition, fat percentage, adiposity index, blood pressure, leptin, adiponectin, plasma, and tissue lipid profiles.
Improves the activity of enzymes involved in lipid metabolism, such as lipase, amylase, HMG-CoA reductase, fatty acid synthase, lipoprotein lipase etc.
Decreases oxidative stress
Shows improvement in glucose tolerance
Supports liver function

Both pepper extract and capsaicin can positively influence human health and treat several disorders. Moreover, they can reduce fat storage affecting brain centers responsible for the sensation of hunger, nutrient uptake by gastrointestinal tract, state of adipocytes, increase in carbohydrate and fat oxidation, metabolism and thermogenesis and other mechanisms. Therefore, despite some possible limitations, these substances could be useful for treatment of obesity [23].

The findings indicate that piperine inhibits the differentiation of fat cells, offering a potential treatment for obesity-related conditions [24].

Studies show that black pepper extract has stronger effects than piperine alone.

There could be a target for treating obesity and type 2 diabetes: raising the consumption of calories by an increase in the metabolic rate of resting skeletal muscle. Piperine increased ATPase activity of skinned relaxed fibers by 66 ± 15%. Piperine had little effect on the mechanics of either fully active or resting muscle fibers. The results described here show that up-regulation of resting muscle metabolism could treat obesity and type 2 diabetes and that piperine would provide a useful lead compound for the development of these therapies

It has been found that, by increasing the calorie consumption in the resting skeletal muscle, leptin resistance, insulin resistance, obesity and type 2 diabetes may be benefit from a very effective treatment. Piperine has been shown to boost ATPase activity in relaxed muscle fibers by 66 ± 15% (!!!). That’s a lot! [25]. What does that mean? It means that, if you manage to boost your basal metabolism (meaning the body’s metabolism when resting – in other words, your muscle metabolism when relaxed).

THIS IS THE WHOLE PURPOSE OF OUR NUTRITIONAL PROTOCOL, the Master AMPK/mTOR Protocol – TO OPTIMIZE YOUR MUSCLES IN ORDER TO BURN MORE FAT WHEN SELLPING!

3. Cayenne pepper extract

Red pepper is part of the Solanaceae family within the Capsicum genus, which is recognized by various names worldwide, including hot pepper, chili pepper, bell pepper, sweet pepper, and sometimes simply pepper.

Red pepper contains natural compounds known as capsaicinoids. Studies have shown that capsaicin and dihydrocapsaicin together make up approximately 70–90% of these capsaicinoids found in the Capsicum genus.

Evidence indicates that red pepper, together with its active ingredients, capsinoids, displays enhanced benefits in promoting energy expenditure (energy usage), including limiting energy intake, which is likely to reduce fat mass in overweight and obese individuals [26].

Capsaicin, a well-known vanilloid, which is the main spicy component in chili peppers, shows several biological activities and the potential applications range from food flavorings to therapeutics. Capsaicin also plays a crucial role in metabolic disorders, including weight loss, blood pressure lowing and insulin reduction effects [27].

Capsaicin is the molecule gives the pungency of hot peppers. It stimulates the sympathoadrenal system, which lead to thermogenic and anorexigenic effects. Capsaicinoids have been found to accentuate the impact of caloric restriction on body weight loss. They are shown to prevent the increase in hunger, enhances satiety, energy expenditure and fat oxidation – which are normally achieved in cases like energy restriction [28].

4. Garlic extract

Garlic (Allium sativum L.) has been used as a traditional remedy in various cultures worldwide for thousands of years, dating back over 6,000 years in Ayurvedic medicine, over 3,000 years in ancient Chinese medicine, and noted in the ancient Egyptian Codex Ebers.

In modern times, garlic has gained popularity as a natural remedy with proven health benefits and therapeutic effects, while causing almost no side effects. It is valued for its flavor and medicinal properties, attributed to its rich concentration of organosulfur compounds.

When garlic is crushed or cut, alliinase enzyme triggers the formation of volatile sulfur compounds. This process gives birth to various reactive sulfenic acids, which then combine to form thiosulfinates. These are the ones which give garlic its distinctive flavor and pungency, “allicin” being the main thiosulfinate produced from the breakdown of alliin.

The bioactive compounds achieve their activities within the gastrointestinal tract (in situ). These compounds need to be bioaccessible but not necessarily be absorbed into the body. Their absorption rates are higher than 90%.

Studies support the beneficial impact of garlic or its sulfur-containing components on obesity and metabolic syndrome. These compounds derived from garlic demonstrate an anti-obesity effect by inhibiting the differentiation of fat cells through mechanisms such as AMPK activation and other mechanisms [29].

Moreover, research provide substantial evidence for the potential of garlic to lower blood pressure (antihypertensive), regulate blood sugar levels (antidiabetic), reduce obesity (fat burning), and reduce lipid levels in the blood (hypolipidemic) [30].

Co-boosters:

1. Green coffee bean extract

Green coffee is famous for its slimming effect. It’s not that long ago when green coffee was a superstar in this area.

The active compounds found in green coffee beans which contribute to weight loss are known as chlorogenic acids (CGA). There are three major classes of chlorogenic acids present in green coffee beans, namely: caffeoylquinic acid, di-caffeoylquinic acid and feruloylquinic acid.

Another pivotal component of the green beans is caffeic acid.

Green coffee upon roasting develops its aromatic characteristics, but the flavor development comes at a cost: reduced chlorogenic acid content. Thus, the effect of green coffee is highly superior to roasted coffee for the purpose at hand. There are numerous studies which show health benefits of green coffee in disorders like hypertension, diabetes, obesity, etc. [31]

According to one meta-analysis, green coffee bean extract with 500 mg/day of chlorogenic acid lowers body weight [32]. (We remind you that we have another 17 ingredients in the formula for this purpose.)

Research demonstrated notable decreases in body weight, fat mass, and waist-to-hip ratio, alongside significant enhancements in lipid profile. This included reductions in total cholesterol, LDL, and leptin levels, along with an increase in serum adiponectin concentration. These results indicate that combining green coffee bean extract with a calorie-controlled diet can be effective in managing weight and enhancing lipid metabolism in people with obesity [33].

Administering green coffee bean extract showed positive effects on specific aspects of metabolic syndrome, such as high systolic blood pressure, elevated fasting blood glucose, insulin resistance, and abdominal obesity. Furthermore, supplementation with green coffee bean extract was linked to a reduction in appetite levels [34].

2. Garcinia cambogia extract

Garcinia cambogia, a tropical fruit also known as Malabar tamarind, originated in Southeast Asia, India, and Africa, has gained attention for its potential role in weight management, particularly in obesity [35].

This fruit contains a compound called hydroxy citric acid (HCA), which is believed to inhibit an enzyme called citrate lyase, involved in fat metabolism. By blocking this enzyme, HCA may help prevent the conversion of carbohydrates into fat, thereby reducing fat accumulation in the body. In addition to other compounds, Garcinia cambogia fruits are rich in various amino acids, including arginine, asparagine, glutamine, threonine, glycine, proline, γ-aminobutyric acid, leucine, isoleucine, ornithine, and lysine.

Hydroxy citric (HCA) acid may prevent fat accumulation by:

Reducing or inactivating the fat-producing enzymes [36]
Lowering the activity of proteins which raise the levels of fat-producing enzymes [37]
Increasing levels of fat-burning enzymes
Lowering the production of triglycerides, phospholipids, cholesterol, and fat in the liver [38]

Garcinia cambogia supplements may also bring about a host of other positive effects, like:

Helping to lower cholesterol and triglyceride levels[39]
Supporting diabetes management[40]
Boosting the immune system
Providing antioxidant benefits[41]

Garcinia cambogia is also said to raise serotonin levels, which is a neurotransmitter in the body that helps with feeling good and communication. By this mechanism it might help in lowering appetite, which could theoretically aid in weight loss[42].

See this article for more insights on the subject: https://supplements.selfdecode.com/blog/garcinia-cambogia/

Extracts derived from Garcinia cambogia and components found in the fruit are primarily recognized for their potential to reduce body weight and appetite, and prevent obesity.[43]

Garcinia cambogia fruits contain guttiferone J, a newly discovered compound known for its ability to trigger thermogenesis. This compound is responsible for the anti-obesity effects of Garcinia cambogia and is being explored as a potential treatment for obesity and associated conditions [44].

This ingredient is a very powerful one. When taken on its own, you should be careful not to take too much. In our formula, the dosage is in the known safe zone. Remember it is a very special combination we are using, so there is no need to oveuse none of them.

• • •

Part II done, moving to part III. Now this is the final list of ingredients in Thot Leptin Rx, but as important as any other presented so far. These ingredients that are about to be describe are essential to raise the energy levels ant to keep them leveled-up. This way, you know that all the work that your body has made through the other ingredients and through the lifestyle changes that you make, the transformation stays long-term.

Part III: Energy support (antioxidant, anti-inflammatory and hypocholesterolemic agents)

Made of: Glycine (hydrochloric glycine), L-Glutamine HCL, Ascorbic Acid (vitamin C) 100%, Black currant extract (Ribes nigrum) (Anthocyanidins ≥25%) and Zinc (Zinc oxide).

1. Glycine

Glycine is the simplest amino acid in the human body. Glycine represents 11.5% of the total amino acid content in the human body. Typically, 80% of the body’s glycine is utilized for protein synthesis. It is mostly produced in the liver and kidneys and it is a building block for collagen, creatine, glucose, purine, porphyrins of heme and primary bile salts.

Glycine is also required for glutathione synthesis, along with L-cysteine (also included in the formula). It is estimated that glutathione synthesis represents nearly 38% of the glycine necessary for all the functions mentioned above. Studies have suggested that low glutathione synthesis rate may play a role in insulin resistance and type 2 diabetes. Glutathione concentration was 60% lower in erythrocytes of patients with type 2 diabetes compared to healthy individuals [45].

Plasma glycine level is consistently lower in patients with obesity and type 2 diabetes compared to healthy persons. Some studies suggest that low plasma glycine level precedes the clinical onset of type 2 diabetes [46].

Supplementing with glycine improves several aspects of metabolic syndrome, which includes diabetes, obesity, hyperlipidemia and hypertension. In the future medical approaches, the use of glycine may be an important option for individuals with metabolic syndrome [47].

At the appropriate dosage, glycine has proven highly effective in reducing not only metabolic disorders but also various inflammatory diseases and cancers [48].

In central nervous system, glycine plays a crucial role as neurotransmitter, regulating food intake, behavior and body homeostasis. It can have both activate or calm the brain and nervous system.

And we get back to communication: between cells and between body and the brain.

Furthermore, glycine helps support the immune system, fights inflammation, and has an antioxidant role in the body.

2. L-Glutamine

L-Glutamine is an amino acid, non-essential, widely distributed in the body, participates in numerous metabolic processes. It’s synthesized from glutamic acid and ammonia. The enzyme responsible for this process is known as glutamine synthetase. Patients with obesity or diabetes show altered glutamine metabolism, including decreases in glutamine [49].

Glutamine can serve as a nitrogen supplier in the production of various compounds, such as other amino acids, purines, and pyrimidines. L-glutamine enhances the redox potential of nicotinamide adenine dinucleotide (NAD) – a very powerful antioxidant[50]. As a supplier of glutamate, it plays an important role in maintaining cellular volume, amino acid balance, glutathione levels and energy production.

Plasma glutamine serves as the precursor for 80% of plasma citrulline, and plasma citrulline, in turn, acts as the precursor for 10% of plasma arginine. Ultimately, glutamine emerges as a significant precursor for arginine synthesis through its conversion to citrulline in the intestines of humans[51].

L-glutamine is a conditionally essential amino acid and main precursor for the production of both glutamate and GABA neurotransmitters in the brain.

All the mechanisms mentioned above lead to an additional support of L-Glutamine in weight loss and energy management, trough pathways that have been already and partially described.

3. Ascorbic acid

It’s suggested that ascorbic acid, or vitamin C, could offer several benefits for obesity-related issues. These include possibly regulating fat burning in fat cells, controlling the release of stress hormones from the adrenal glands, and even regulating sugar metabolism, including leptin secretion, and reducing inflammation. Studies show it might help improve blood sugar levels and reduce complications in obese individuals with diabetes. These effects are likely tied to its powerful antioxidant properties [52].

Having antioxidant capabilities, vitamin C protects against oxidative stress and prevent non-enzymatic glycosylation of proteins (which has been described above). Moreover, it reduces lipid peroxidation and inflammation. The anti-inflammatory attribute of vitamin C may serve as a protective factor in metabolic diseases [53].

4. Black currant extract

While research on this specific topic is limited, black currant extract is known to contain various bioactive compounds, including anthocyanins, flavonoids, and polyphenols, which have antioxidant and anti-inflammatory properties.

Studies show that black currant extract reduces inflammation. The metabolites of blackcurrant may not have anti-inflammatory effects directly by altering macrophage phenotypes, but by modulating levels of obesity-induced circulating pro-inflammatory factors [54].

Enzymatic extract’s high antioxidant activity was likely attributable to its high concentration of anthocyanin. Black currant extract inhibits lipid accumulation in adipocytes [55].

Black currant’s antioxidant composition might contain traces of chromium picolinate, which is known for aiding in glycemic control for individuals with diabetes and promoting the maintenance of lean body mass[56].

Whole berry and juice is rich in compounds like Cyanidin 3-glucoside, cyanidin 3-rutinoside, delphinidin 3-glucoside, delphinidin 3-rutinoside, and ascorbic acid and are shown to have the following health benefits, which can contribute to a healthy metabolism and energy management[57]:

has an antioxidant effect
shows an anti-inflammatory effect
lowers cholesterol and improves metabolic functions
increases fat oxidation

5. Zinc oxide

Zinc (Zn) is known for its wide range of benefits in human health, from antioxidant properties to almost any mechanism you can think of.

Zinc (Zn) is vital in biological processes, influencing gene transcription, cell signaling, enzyme function, and the regulation of metabolism and brain activity. It’s recognized for its contribution to insulin synthesis, storage, secretion, and sensitivity. In the brain, zinc is involved in synaptic plasticity and acts as a neurotransmitter, participating in both intra- and intercellular processes. Zn supplementation was associated with a significant reduction in body weight in overweight or obese individuals ^[58].

The scientists tried to find a more efficient way to deliver the Zinc oxide, and they discovered that Zn oxide nanoparticles are a very good solution. Studies now show that Zn oxide nanoparticles significantly decreased both high-fat diet induced hepatic steatosis and peripheral insulin resistance. These indicates that Zn can serve as a therapeutic strategy to improve the physiological homeostasis in obesity and its associated metabolic abnormalities [59].

Benefits of Thot Leptin Rx

Thot Leptin Ex is formulated to help improve the body’s natural metabolic functions and to help overweight and obese people to lose weight. This is achieved through 3 basic and vital mechanisms:

It improves leptin sensitivity and overcomes leptin resistance
It boosts metabolism & rises energy level in order to burn more fat
It restores and improves the brain-body and intercellular communication

Overall, trough all these mechanisms, Thot Leptin Rx contributes to the normal metabolism of carbohydrates, fatty acids, and macronutrients, as well as to normal protein synthesis, acid-base balance, and energy metabolism.

Thot Leptin Rx’s complex and unique formulation holds and approaches multiple aspects and mechanisms of weight management and metabolic health and offers a holistic approach to achieving and maintaining an ideal and healthy weight.

Usage and Dosage

In order to maximize the effectiveness of Thot Leptin Rx in leptin resistance and weight loss, we recommend a daily dose of 4-6 tablets/day with meals or with liquids.

The tablets can be swallowed with an appropriate quantity of water or they can be chewed or even enjoyed while dissolving in the mouth. Due to the spices in the formula, the tablets in Thot Leptin Rx taste similar to a “Thai soup”.

A dietary supplement does not replace a balanced diet and e healthy lifestyle. In you have any health condition, please consult your medical doctor or your health specialist.

Conclusion

Overcoming leptin resistance is THE KEY for achieving long-term success in weight management and depends on boosting the basic metabolic rate and restoring the communication within the whole body.

In 2024, Thot Leptin Rx is THE ONLY SUPPLEMENT which holds THE SOLUTION to optimize all the mechanisms involved in weight management.

Learn more about Thot Leptin Rx and consider incorporating it into your weight loss journey, particularly if you struggle with leptin resistance and you want fast, but long-lasting results.

Thot Leptin RX by Gabriel Pesa aka Bralgei Shackry is licensed under Creative Commons Attribution-ShareAlike 4.0 International

Thot Leptin RX by Gabriel Pesa aka Bralgei Shackry is licensed under Creative Commons Attribution-ShareAlike 4.0 International

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L-Lysine the best and only key to collagen and healthy body in 2023

ThotNutrition2025 — Mon, 13 Nov 2023 10:07:22 +0000

1. Introduction

If you want collagen, beauty, skin regeneration, anti-aging, your answer is very simple: L-Lysine and essential amino acids (Thot Aminos) – the best and only way… Then you can skip the science.

In order to produce collagen, you need a lot of L-Lysine and vitamin C
Bralgei Shackry aka Gabriel Pesa

L-Lysine the key to collagen and healthy body

Is that all? Yes. Your body can produce collagen if you have these 2 ingredients.

We give you the highest grade and the highest concentration of L-Lysine on the market.

You take care of the vitamin C

…now let’s go into details and science

L-Lysine the key

Of all the 9 essential amino acids, L-Lysine is a special one when it comes to human body structure, beauty and cosmetics (skin, tendons, blood vessels, cartilages, bones and connective tissue – which anchors cells to each other).

Yes, I’m talking about L-Lysine because it is known especially for collagen synthesis, skin and for stabilizing the structural matrix. Collagen is the most abundant protein in our body[1]. It represents between 25-35% of all proteins and 75% of skin. There is an entire beauty industry . However, few people know the real science behind the production, regeneration and long-time maintenance of the matrix.

There are 28 known types of collagen the most abundant being type I – 90% (skin, tendon, vasculature, organs, bone), type II (cartilage), type III (reticular fibers) and type IV (basement membrane).

The story around collagen is pretty long and complicated, but here you have the opportunity to get the most important conclusions after years of studying and learning about such an important protein. Two of the key essential elements are L-Lysine and Vitamin C. One is an essential amino acid and the other is an essential vitamin. Neither one can be produced by the body. Skin and anti-aging is depending on them.

Don’t you find it a little odd that the synthesis of the most abundant protein in the body depends on two elements that the body cannot produce? Have you ever asked yourself why? I might be wrong, but… depending on an outside nutrient might be one of the Universe’s elegant ways to show to the arrogant human being that he is still part of something bigger than himself.

Coming back to collagen, there are a lot of hydrolyzed forms that are being sold in the entire world. The question is: are supplements really effective? There is a huge debate around this topic: some studies say they work, some say they are not that effective. If you want to see more on this matter, I highly encourage you to read some articles:

Cosmetics industries understood the importance of L-Lysine in skin health and care. Doctors around the world shared their view on L-Lysine and its recognized skincare benefits[2]. However, L-Lysine is not the only factor in this equation. One must not rely on one supplement for its rejuvenating properties. Vitamin C is too a key element, but a proper cellular hydration and a healthy lifestyle is a must. Then we can talk supplements.

L-Lysine is the most effective essential amino acid in skin rejuvenation and it significantly improves our appearance. But L-Lysine does much more than that when it comes to overall health. Together we will discover the details about L-Lysine and its functions, benefits and uses.

Take your time and enjoy reading our article!

2. L-Lysine and Essential Amino Acids in Health, Body Structure and Beauty

L-Lysine is the most important beauty-oriented amino acid of all the 9 essential amino acids (EAAs). This amino acid has many essential roles in various body functions. All of these roles and pathways will be presented in detail. But first, let’s recap:

L-Lysine the key to collagen and healthy body

2.1. What are Essential Amino Acids?

Amino acids are the building blocks of life[3] – they are indispensable to life – the ones the body uses to build up proteins, hormones, neurotransmitters and all that is necessary.

There are 20 amino acids in nature, of which 9 are essential amino acids (they must be supplemented from food, as the human body cannot synthesize them): L-Leucine, L-valine, L-isoleucine, L-tryptophan, L-phenylalanine, L-methionine, L-lysine, L-threonine, L-histidine

Amino acids and enzymes are the key supplements that underlie the optimal functioning of protein metabolism. This is the indispensable team for the human body performance. The reality is that we are all deficient in amino acids. For the curious people, there are test panels for amino acid deficiencies[3].

Amino acids are used for protein synthesis. Proteins are really the molecules that keep our body alive and running. Life would not exist without proteins. And life thrives when we have the right proteins. Most people believe that it is proteins that we need to take out from our diet. I love to break it to you: it’s not proteins, it’s an amino-acids that we need.

The body cannot absorb proteins. They are digested by enzymes and broken down into amino acids. They are absorbed and used to build the appropriate proteins for the human body. I repeat. It’s the amino acids that we need.

2.2 Why ESSENTIAL (INDISPENSABILE) Amino Acids?

As I’ve already mentioned above, essential amino acids are those 9 amino acids that the human body, in the evolutionary process, has lost the genetic ability to synthetize. Therefore, they need to be supplemented from an outside source.

These are the 9 essential amino acids – their roles in the human body:

L-Leucine – stimulates muscle protein synthesis, being directly involved in the functions of anabolism or body growth and development[5].
L-Lysine – is involved in the most important regulatory processes like gene expression, cellular metabolism and protein stability. Practically, L-Lysine is the key to epigenetics[6] and is essential in longevity and regeneration.
L-Isoleucine – is an important regulator of blood sugar, muscle repair and energy regulation[7].
L-Valine – is both an EAA and a BCAA. It is needed for muscle, tissue repair and maintaining an optimal level of nitrogen in the body. Along with leucine and isoleucine, it helps protect muscle tissue[8].
L-Threonine – is important for the production of antibodies, for the immune system and can be converted to glycine and serine. It also contributes to the synthesis of collagen, elastin and tooth enamel and helps in liver metabolism[9].
L-Phenylalanine – stimulates the synthesis and release of neurotransmitters and hormones such as dopamine and norepinephrine, substances necessary for the optimal activity of the Central Nervous System and the Peripheral Nervous System. They are basically the two neurotransmitters that make us “feel good” and get over an intense pain or an unpleasant event more easily[10].
L-Methionine – provides the body with sulfur – sulfur is very important in the synthesis of glutathione – the most powerful antioxidant in the body, the synthesis of cysteine, taurine, as well as other compounds necessary for optimal functioning, growth and regeneration, detoxification[11].
L-Histidine – previously considered non-essential for adults, histidine is involved in important mechanisms involving the immune, inflammatory and digestive systems. It is a precursor for a powerful antioxidant and for an effective UV light protector[12].
L-Tryptophan – helps release basic neurotransmitters, hormones for well-being and sleep. Examples include serotonin (the “hormone of happiness”) and melatonin (the hormone that regulates the sleep-wake cycle)[13].

“It is not protein that we need, it is amino acids:

essential, bio-available, orthomolecular (left-folded)”

Thot Aminos New Bottle – Golden

Read more about essential amino acids by accessing the main article:

Building blocks of life and nutrition – What are essential amino acids?

To find out more about the muscle – the organ of longevity – please read our article L-Leucine – the best key to muscle recovery, health and longevity in 2023,

2.3 L-Lysine – The Most Important Essential Amino Acid for Structural Integrity and BEAUTY

To begin with, L-Lysine, like all the other amino acids in Thot Aminos formula, is an essential amino acid, therefore it needs to be supplemented from an outside source: foods rich in L-Lysine or efficient supplements. The body cannot produce essential amino acids, but they are all vital for the body to accomplish its functions.

Thot Nutrition offers the best formula with the highest quality ingredients.

For biohacking style, we bring to you the best L-Lysine in the world – vegan, pure, orthomolecular, low molecule, high density, low molecule, top quality – ready for direct absorption and utilization.
Bralgei Shackry

L-Lysine the key to collagen and healthy body

Known for its beauty effects, L-Lysine is essential for collagen synthesis, but has multiple roles. The main ones are as it follows:

Functions and Benefits of L-Lysine:

It contributes to collagen synthesis and stabilization of the collagen matrix. Two of the key essential elements in collagen synthesis are L-lysine and vitamin C. One is an essential amino acid and the other is an essential vitamin. None can be produced by the body and need to be taken from outside.
Collagen is an essential structural protein that provides strength, elasticity and support to many tissues, including skin, tendons, ligaments and blood vessels. It’s what actually holds our cells together, in a structured and organized form! Without a solid collagen matrix we would be nothing more than a mass of cells of different shapes and sizes.
Participates in protein synthesis. L-lysine helps decode information stored in mRNA during the translation step, along with other amino acids. Apart from that, it is an essential amino acid that participates in the structure of proteins.
Supports muscle growth and recovery. Intense workouts put a lot of strain on the body, and an optimal collagen matrix ensures strong connective tissues, reducing the risk of injury. In addition, L-lysine facilitates the production of nitric oxide, known for vasodilation and oxygenation of muscles.
Improves liver function and lowers cholesterol levels. L-lysine is an essential amino acid involved in the production of digestive and metabolic enzymes that optimize triglyceride and cholesterol levels regulated by the liver.
Improves calcium absorption. L-Lysine improves calcium absorption from the intestines and potentiates the effects of vitamin D. By forming a strong collagen matrix in bones, calcium is retained in bone tissue in a greater proportion.
Strengthens the immune system. L-Lysine has significant antiviral activity, especially against simple herpesvirus HVS. It has immunomodulatory effects and stimulates the production of antibodies.
It can help manage herpes outbreaks. L-lysine has the ability to reduce the frequency, severity and duration of herpes outbreaks. The HSV virus requires the amino acid arginine to replicate. L-lysine and arginine compete for the absorption pathway, and it is thought that increasing lysine levels and reducing arginine levels may help suppress viral replication.[4]
Helps in the synthesis of hormones. L-lysine has the potential to stimulate the release of growth hormone from the pituitary gland. Growth hormone is essential for growth, development and maintenance of optimal body composition. L-lysine may also play a role in the synthesis of serotonin, known as the “hormone of happiness,” as well as other hormones, like all other essential amino acids.
Supports optimal brain activity. L-lysine is involved in the synthesis of various neurotransmitters, such as serotonin, dopamine and norepinephrine, involved in good mood and pleasure. Optimal levels of lysine contribute to dopamine production, supporting cognitive function, concentration, and motivation. It oxygenates the brain by stimulating nitric oxide synthesis and reducing inflammation and oxidative stress.
Maintains cardiovascular health. By engaging in collagen synthesis, L-lysine helps maintain strong , elastic and resilient blood vessels. In addition, it has the potential to reduce arterial plaque formation, known as atherosclerosis. Studies have also shown that L-lysine supplementation can help lower blood pressure levels.
Regulates gene expression.5]

These pathways all contribute to a healthy and beautiful body, especially the one involving collagen synthesis. We will now break down these mechanisms for you to better understand how exactly can L-Lysine significantly improve your body’s appearance in a way that is not only beautiful, but natural, healthy and long-lasting. Who wouldn’t want a truly long-lasting effect??

[1] Di Lullo GA, Sweeney SM, Korkko J, Ala-Kokko L, San Antonio JD. Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen. J Biol Chem. 2002 Feb 8;277(6):4223-31.

[2] Byrdie – Lysine for Skin

[3] Building blocks of life and nutrition – What are essential amino acids?

[4] Thein DJ, Hurt WC. Lysine as a prophylactic agent in the treatment of recurrent herpes simplex labialis. Oral Surg Oral Med Oral Pathol. 1984 Dec;58(6):659-66.

[5] Dambacher S, Hahn M, Schotta G. Epigenetic regulation of development by histone lysine methylation. Heredity (Edinb). 2010 Jul;105(1):24-37.

2 How does L-Lysine improve our body?

This will be an interesting journey.

2.2 L-Lysine is a very important ESSENTIAL amino acid

L-Lysine cannot be produced by the body and must be obtained through diet.
In general, it is best to consume EAAs in a balanced and varied diet. However, for individuals who may not be able to consume and/or extract enough EAAs through diet alone, supplementation is key. It is important to consult with a healthcare provider before starting any supplementation regimen.
There are various L-Lysine rich foods. However, as you may have already read in our main article, the human body generally has trouble digesting, extracting, absorbing, folding and using essential amino acids, including L-Lysine. This comes with aging and appears due to the lack of enzymes – The already “famous” enzymes-amino acids vicious cycle (at least on this website).
Here you can find a list of L-Lysine rich foods. L-Lysine is naturally present in the following foods like[1]:
Beef & lamb meat – 3582 mg/100g
Parmesan – 3306 mg/100g
Poultry meat (turkey or chicken) – 3110 mg/100g
Pork meat – 2757 mg/100g
Soy beans & products – 2634 mg/100g
Tuna fish – 2590 mg/100g
Shrimp – 2172 mg/100g
Pumpkin seeds – 1386 mg/100g
Eggs – 912 mg/100g
White beans – 668 mg/100g
Other fish (cod, sardine) and shellfish
Spirulina and fenugreek seeds

Sure… you can use food as a source for your aminos… but…

For biohacking style, we bring to you the best L-Lysine in the world – vegan, pure, orthomolecular, low molecule, high density, low molecule, top quality – ready for direct absorption and utilization.

3.2 L-Lysine’s FUNCTIONS in the Human Body

3.2.1 L-Lysine supports COLLAGEN FORMATION

Collagen is an essential structural protein that provides strength, elasticity, and support to many tissues, including the skin, tendons, ligaments, and blood vessels. It’s what actually keeps our cells together, in a structured and organized form! Without a solid collagen matrix, we would be nothing but a mass of cells with different shapes and sizes. Long story short, if you manage to optimally and constantly regenerate your collagen matrix, youth and beauty is nothing but a natural consequence! Can it really be done? With the right knowledge and practice, anything can be done!

Collagen synthesis is not a simple process. Moreover, stabilizing the collagen structure is at least as important as its synthesis. To read more on collagen synthesis, please read.

So, let’s see how L-Lysine supports the collagen matrix:

Firstly, L-Lysine is involved in a critical step of collagen synthesis, known as hydroxylation[2]. The addition of hydroxyl groups to some amino acids, such as proline and lysine, provides stability and proper folding of collagen molecules.

Then, L-Lysine helps in cross-linking of collagen fibers[3]. After collagen molecules get out of the cells, there are some enzymatic cross-links between collagen molecules. These cross-links make the collagen fibers stronger and more stable.

L-Lysine contributes to collagen’s stability and structure[4], participating in the formation of hydrogen bonds, electrostatic interactions, and other chemical interactions within and between collagen molecules. All of these helps maintain the triple helical structure of collagen and a proper alignment and organization.

Collagen is known to be synthesized in wound healing, in order to replace damaged or lost tissue. For the collagen production during the wound healing, the body needs sufficient amounts of L-Lysine.

It is now obvious that L-Lysine supports skin health and elasticity. Collagen is a major component of the skin and is responsible for its strength, elasticity, and resilience. Besides the structural support, collagen helps retain moisture, and contribute to the skin’s elasticity.

3.2.2 L-Lysine promotes PROTEIN SYNTHESIS

Protein synthesis is a vital process that involves the creation of new proteins from amino acids. The detailed mechanisms through which L-Lysine participates in protein synthesis are the following:

First and foremost, L-Lysine is an Essential Amino Acid. All essential amino acids must be obtained through the diet, one way or another. Without an adequate supply of L-Lysine or any other essential amino acid, the body cannot synthesize proteins properly. Remember! Proportion is the key! Not too long ago, the egg protein was considered to be the ideal protein for human consumption. More recent studies suggest there can be an even better proportion. The quest for the perfect formula led to the making of Thot Aminos.

Protein synthesis is done through several steps. In the first stage, the genetic information stored in your DNA is transcribed into messenger RNA (mRNA), a process called transcription. Then, the mRNA carries the instructions to the ribosomes, where the information stored in the mRNA is decoded, a process called translation. L-Lysine helps in decoding the information stored in the mRNA during translation, together with other amino acids.

During protein synthesis in the ribosomes, L-Lysine is attached to transfer RNA (tRNA) molecules, forming aminoacyl-tRNA. This compound carries L-Lysine to the ribosomes and it is then matched with the specific codon for L-Lysine in the mRNA sequence, ensuring the correct incorporation of L-Lysine into the growing protein chain. In other words, L-Lysine has to get to a very specific place in the protein chain in order for the protein to be correctly structured and functional, and this is ensured by aminoacyl-tRNA formation.

Last, but not least, L-Lysine participates in peptide bond formation. L-Lysine contributes to the elongation of the protein chain by forming peptide bonds with neighboring amino acids.

3.2.3 L-Lysine supports MUSCLE GROWTH and RECOVERY

L-Lysine’s role in protein synthesis makes it particularly beneficial for muscle growth and recovery. During exercise or physical activity, muscle tissues experience wear and tear, requiring repair and regeneration. However, when we talk about muscle growth, we talk really about the whole THOT AMINOS formula and especially about Thot Nutrition’s top quality L-Leucine.

See also the best L-Leucine on the market. Is a co-factor in protein synthesis.

But, in practice, weightlifting and other physical activities demand very strong connective tissues. L-Lysine’s support in collagen synthesis indirectly aids in my muscle growth by maintaining their integrity and reducing the risk of injuries.

In addition to that, after intense workouts, the body’s immune system can be temporarily compromised. L-Lysine’s role in immune support indirectly helps you stay consistent with your training, without worrying about any illness.

Furthermore, the nitric oxide production facilitated by L-Lysine enhances muscle pump and blood flow during workouts. This increased blood flow delivers more oxygen and nutrients to the muscles, providing an extra boost for growth and aiding in post-workout recovery.

Lastly, L-Lysine’s antioxidant properties have been valuable in combating the oxidative stress induced by intense exercise, allowing faster recovery for the muscles. However, remember that, when it comes to muscle, L-Lysine may help, but you need all 9 essential aminos, especially L-Leucine.

3.2.4 L-Lysine enhances CALCIUM ABSORBTION, RETENTION & UTILIZATION [5]

Calcium is an essential mineral that is crucial for various bodily functions, including bone health, muscle contractions, nerve transmission, and blood clotting. L-Lysine ensures that an adequate amount of calcium is available for these functions.

Lysine has been found to enhance calcium absorption in the intestines: L-Lysine works hand in hand with calcium transporters in your body, ensuring that calcium from your diet gets absorbed efficiently into your bloodstream.

As you surely know by now, L-Lysine is involved in collagen synthesis, the protein that forms the structural framework of your bones. When collagen is strong, it helps retain calcium within the bone matrix. Bones are made of 60% collagen and 40% a mix of 12 minerals including (Boron, Calcium, Chromium, Iron, Magnesium, Manganese, Selenium, Sodium, Potassium, Sulfur, Silica and Phosphorus) plus 65 trace minerals, including. So, by supporting collagen production, L-Lysine contributes to maintaining healthy calcium levels in your bones.

L-Lysine also plays a role in the synthesis of calcium-binding proteins, which help regulate the levels of calcium within your cells. These proteins ensure that calcium is properly utilized in various cellular processes throughout your body.

Few know that L-Lysine and vitamin D work together to optimize calcium absorption. Vitamin D helps regulate calcium levels and promotes its absorption from the intestines, while L-Lysine enhances the effects of vitamin D on calcium absorption.

Maintaining a rich ionic calcium diet is done through consuming leafy green salads. Green leaves store enough calcium, without needing to supplement it. It is more important to regulate calcium metabolism than to supplement with calcium!

3.2.5 L-Lysine boosts IMMUNE FUNCTION

Lysine plays a significant role in supporting a healthy immune system. It is involved in the production of antibodies, which are proteins that help identify and neutralize foreign invaders such as bacteria, viruses, and other pathogens.

This essential amino acid has been studied for its potential antiviral properties, particularly against certain types of viruses, including the herpes simplex virus (HSV)[6].

Adequate levels of L-Lysine are necessary for the optimal functioning of immune cells like lymphocytes and natural killer (NK) cells, which are responsible for recognizing and eliminating pathogens.

L-Lysine has been shown to have immunomodulatory effects, regulating the production of certain immune factors, such as cytokines. This modulation helps in preventing excessive inflammation or immune overactivity.

And last but not least, L-Lysine supplementation has been found to reduce the effects of stress, by reducing cortisol levels[7]. Stress can weaken immune function, making you more susceptible to infections. L-Lysine may help counteract some of the negative impacts of stress on the immune system.

3.2.6 L-Lysine may help manage viral and HERPES infections

Lysine has gained attention for its potential role in managing outbreaks caused by the herpes simplex virus. Let’s see what are the biological and molecular mechanisms involved in this.

Herpes Simplex Viral Infections: HSV-1 is primarily responsible for cold sores (oral herpes), while HSV-2 is associated with genital herpes. L-Lysine has been investigated for its ability to reduce the frequency, severity, and duration of outbreaks in these conditions[8].

HSV requires the amino acid arginine to replicate. L-Lysine and arginine share a common pathway for absorption, and it is believed that increasing the ratio of lysine to arginine can help suppress viral replication. L-Lysine competes with arginine for absorption and utilization, potentially limiting arginine’s availability for viral replication. By increasing lysine levels and reducing arginine levels, lysine supplementation may help inhibit viral replication and reduce the frequency, duration, and severity of herpes outbreaks, including cold sores[9].

Even if some studies suggest potential benefits of L-Lysine for herpes management, results may vary from person to person. It’s recommended to consult with a healthcare professional for personalized advice.

3.2.7 L-Lysine assists in HORMONE PRODUCTION

L-Lysine has been studied for its potential to stimulate the release of growth hormone from the pituitary gland. Growth hormone is essential for growth, development, and maintaining healthy body composition. L-Lysine supplementation has been found to enhance the effects of growth hormone-releasing hormone (GHRH), which may contribute to increased growth hormone secretion.

In my opinion, stimulating HGH is the most important factor for antiaging corelated with L-Lysine.

L-Lysine and arginine share a competitive relationship for absorption and cellular uptake. Studies suggest that maintaining an appropriate balance between lysine and arginine levels may impact certain hormone pathways. For example, lysine can inhibit arginine-induced insulin release, potentially influencing insulin regulation in the body.

Moreover, L-Lysine may play a role in serotonin synthesis. Serotonin is a neurotransmitter and hormone that regulates mood, appetite, and sleep. A deficiency in lysine could potentially affect serotonin levels.

Please consult a healthcare professional before supplementing with L-Lysine, particularly when talking about hormones.

3.2.8 L-Lysine supports BRAIN HEALTH

L-Lysine is involved in the synthesis of various neurotransmitters such as serotonin, dopamine, and norepinephrine. Adequate lysine levels help maintain balanced serotonin levels, promoting a positive mood and emotional well-being[10], while dopamine is a neurotransmitter associated with reward, motivation, and pleasure. Optimal lysine levels contribute to dopamine production, supporting cognitive function, focus, and motivation.

Moreover, scientific evidence suggests that the brain’s nitric oxide (NO) signaling system may be involved in schizophrenia and could thus constitute a treatment. The study was designed to investigate the benefit of L-lysine, which interferes with NO production, as an add-on treatment for schizophrenia[11].

Having antioxidant properties, L-Lysine can help neutralize harmful free radicals in the brain. By reducing oxidative stress and health by reducing inflammation and immune-related damage, L-Lysine may help protect brain cells in cognitive decline and neurodegenerative diseases.

3.2.9 L-Lysine helps maintain CARDIOVASCULAR HEALTH

Cardiovascular disease is one of the top health problems worldwide. It is only natural for healthcare practitioners to search for natural and effective solutions to help in this area. Although L-Lysine is not the main essential amino acid to improve cardiovascular health, it has some benefits worth mentioning. Since the heart muscle is a muscle, for muscle and cardiovascular health, please read our article L-Leucine – the best key to muscle recovery, health and longevity in 2023,

By being involved in collagen synthesis L-Lysine indirectly provides structural support to blood vessels. Collagen helps maintain strong, elastic, and resistant blood vessels. Healthy blood vessels have a major contribution to cardiovascular health, especially when we talk about hypertension.

L-Lysine has also been studied for its potential to reduce the formation of arterial plaque, known as atherosclerosis. L-Lysine may prevent the accumulation of lipoprotein, a type of cholesterol associated with increased risk of cardiovascular disease.

Studies have also shown that L-Lysine supplementation can help lower blood pressure levels, particularly in individuals with hypertension[12]. Therefore, the risk of cardiovascular events such as heart attacks and strokes may be decreased.

In general, whenever we talk about chronic disease, we talk about chronic inflammation. The cardiovascular system is no exception. Known to reduce inflammation, L-Lysine may help prevent heart disease and protect the heart and vessels. L-Lysine is also known for its antioxidant effects, which can help neutralize free radicals and reduce oxidative stress in the cardiovascular system.

L-Lysine is an essential nutrient which can support cardiovascular health, that’s true. However, do not neglect your cardiovascular care provided by your medical practitioner, nor a healthy diet or lifestyle. When it comes to the heart, it’s serious business.

3.2.10 L-Lysine and EPIGENETICS (Regulating GENE EXPRESION)

There isn’t substantial evidence to suggest that L-Lysine directly regulates gene expression in a well-established and direct manner, but there are studies that suggests this:

Enzymes of Epigenetics, Part A

D. Hayward, P.A. Cole, in Methods in Enzymology, 2016

Abstract:

The lysine-specific demethylase (LSD1) is a flavin-dependent amine oxidase that selectively removes one or two methyl groups from histone H3 at the Lys4 position. Along with histone deacetylases 1 and 2, LSD1 is involved in epigenetically silencing gene expression. LSD1 has been implicated as a potential therapeutic target in cancer and other diseases. In this chapter, we discuss several approaches to measure LSD1 demethylase activity and their relative strengths and limitations for inhibitor discovery and mechanistic characterization. In addition, we review the principal established chemical functional groups derived from monoamine oxidase inhibitors that have been investigated in the context of LSD1 as demethylase inhibitors. Finally, we highlight a few examples of recently developed LSD1 mechanism-based inactivators and their biomedical applications.

However, the regulation of gene expression is a complex process involving multiple factors, including transcription factors, epigenetic modifications, and various signaling pathways. While amino acids, including l-lysine, are essential for the synthesis of proteins, they typically do not have a direct role in the control of gene expression at the molecular level.

It’s important to note that scientific understanding evolves, and new research may emerge. If there have been developments in the field of molecular biology or biochemistry after my last update, I recommend checking more recent sources or scientific literature for the latest information on the topic.

3.3 Medical uses for L-Lysine

Although this article is not about medical advice of any kind, although the purpose of this article is in educating the people towards a healthy lifestyle, which naturally prevents disease, we cannot ignore the fact that nutrients like L-Lysine and the others have been discovered to have some medical uses. L-Lysine, the natural form of the amino acid lysine, has several recognized therapeutic applications. These are the main ones:

3.3.1 L-Lysine in Herpes Simplex Virus (HSV) infections:

L-Lysine is commonly used as a dietary supplement to manage outbreaks of herpes simplex virus infections, including cold sores (oral herpes) and genital herpes. Some studies suggest that high-dose lysine supplementation may help reduce the frequency, duration, and severity of outbreaks. Lysine is believed to inhibit the replication of the herpes virus by competing with arginine, which is required for viral replication.

3.3.2 L-Lysine in Osteoporosis:

Lysine plays a role in calcium absorption and collagen synthesis, both of which are important for bone health[13]. Some studies have suggested that lysine supplementation, in combination with other nutrients like calcium and vitamin D, may help improve bone mineral density and reduce the risk of osteoporosis, especially in postmenopausal women.

3.3.3 L-Lysine in Anxiety and Stress

Lysine is involved in the synthesis of neurotransmitters like serotonin, which plays a role in mood regulation. Some evidence suggests that lysine supplementation may help reduce anxiety symptoms and promote a sense of calmness[14].

3.3.4 L-Lysine in Wound Healing:

Lysine’s involvement in collagen synthesis makes it important for wound healing. Collagen is a key component of skin tissue, and adequate lysine levels are necessary for the production and proper arrangement of collagen fibers. Lysine supplementation may help support wound healing, particularly in cases of surgical incisions, burns, and other skin injuries.

3.3.5 L-Lysine in Viral Infections:

Besides herpes viruses, lysine has been investigated for its potential antiviral activity against other viruses, such as human immunodeficiency virus (HIV), Epstein-Barr virus (EBV), and human papillomavirus (HPV). However, more research is required to determine its effectiveness against these viral infections.

While L-Lysine has shown promise in these areas, larger and more high-quality research is needed to establish its effectiveness and determine optimal dosages. Please consult with a healthcare professional before starting L-Lysine supplementation.

4 L-Lysine – KEY ELEMENTS in HEALTH, BODY STRUCTURE & BEAUTY

4.1 The Source – WHY is our L-Lysine no.1 in the world?

One of the most important aspects in the supplement industry, especially when it is about the building blocks of life itself, is THE SOURCE of the supplement. Being pure, with the least possible additives, and the label matching the actual product should not even be mentioned, it should be self-evident. Thot Nutrition has been searching and found the best of the best in every possible aspect of what a product can deliver. What does this mean?
So far, the best L-Lysine suppliers on the market have managed to deliver pretty effective whey-based products. Our L-Lysine is manufactured by the best producers in the world, with instant absorption and utilization. How many products deliver this kind of efficiency? No digestion needed (just dissolving) and no metabolizing needed (already folded properly).
Our L-Lysine is soluble; making is very easy to be absorbed and used by the body with almost no processing.
Our L-Lysine supplement is orthomolecular. This means it is properly folded to the left, so the body can utilize it instantly, without any other process needed prior to utilization.
Our L-Lysine is plant-based. The basic protein source in nature is either plant-based or animals-based. The reality is that animals are just an intermediate organism in the life chain, build to extract essential amino acids from plants and bring it into a single organism. Thot Nutrition understands the natural order of life and uses modern technology to produce and extract all 9 essential amino acids through biosynthesis, directly from plants, avoiding all the unnecessary effects of meat consumption. Most L-Lysine products are whey-based. Animal meat and animal-based products like whey are just an intermediary form of life in the food chain, designed to deliver the whole range of essential amino acids to the human needs. Whey has passed the test of time, but modern technology brings us to the next level: take your essential amino acids directly from the source. Pure, optimized and carefully formulated by Bralgei Shackry (www.bralgei.com ).
Our L-Lysine has a very low molecule. The reduced size allows it to enter places in the body other products could not even dream of. One of the secrets of its efficacy is that it can get to places others cannot, with a very high probability of surpassing the natural protection mechanisms like the blood brain barrier for the brain or the pericardium for the heart.
In addition to that, our L-Lysine is high density, so you have more molecules per dose than all the products currently available on the market.

We are proud to bring Japan quality to our country via our state of art products. We deserve the best.

YOU deserve the best.

4.2 L-Lysine – the “BEAUTY Wizard” of Essential Amino Acids

Type I collagen is the most abundant structural protein in vertebrates. It is composed of two α1 chains and one α2 chain, forming a long triple helix. During biosynthesis, collagen acquires many post-translational modifications, including lysine modifications, critical to the structure and biological functions of this protein. These modifications of collagen are highly complicated[15] and they are catalyzed by several groups of enzymes, all going to the final step of biosynthesis – covalent intermolecular cross-linking of collagen.

L-Lysine has been found to have a synergistic effect when combined with vitamin C. These two nutrients work together to promote collagen synthesis, boost immune function, and support overall health. Vitamin C helps enhance the absorption and utilization of L-Lysine in the body, making them a powerful combination for various health benefits.

When it comes to beauty, L-Lysine can be a helpful ally for your skin, hair, and nails. By supporting collagen synthesis, it can improve the elasticity of your skin, reducing the appearance of fine lines and wrinkles. This amino acid also plays a role in healthy hair growth and strengthening your nails.

If you’re looking to upgrade your beauty routine, you might seriously consider adding L-Lysine into your daily intake. Of course, real and long-term self-care needs a holistic approach: for more details, check out my article on Longevity Practices.

4.3 L-Lysine in REJUVENATION & ANTI-AGING

In addition to collagen synthesis, L-Lysine may be very important in the activation of mTORC1[16].

Mechanistic target of rapamycin complex 1 (mTORC1) is a protein complex that functions as a nutrient/energy/redox sensor and controls protein synthesis activity. It is sensitive to changes in amino acid levels, especially to L-Lysine, regulating cell growth and metabolism.

I have already mentioned the role of the lysine in the production of HGH (Human Growh Hormone) that is essential to regeneration, rejuvenation and youth.

Moreover, there is a L-Lysine containing peptide, called GHK (glycyl-L-histidyl-L-lysine) known to be a natural modulator in skin regeneration. GHK is present in human plasma, saliva and urine but declines with age. GHK coupled with copper may accelerate wound healing and skin repair.

Health & beauty benefits are proposed by recent discoveries as presented below:

stimulates both synthesis and breakdown of collagen
stimulates production of HGH
modulates the activity of both metalloproteinases and their inhibitors
stimulates dermatan sulfate, chondroitin sulfate and decorin (proteinoglycan) in cartilage
restores vitality to fibroblasts after radiation therapy
attracts immune and endothelial cells to the site of an injury
accelerates systemic wound-healing
stimulates hair follicles, gastrointestinal tract, boney tissue

In cosmetic products, it has been found to tighten loose skin and improve skin elasticity, density and firmness, to reduce fine lines and wrinkles, photodamage and hyper-pigmentation, and to increase keratinocyte production. According to recent studies, GHK has could be a therapeutic agent in skin inflammation, COPD and colon cancer. It is known for the capacity to up- and downregulate at least 4,000 human genes, in other words for being capable of resetting DNA to a healthier state [17].

So… knowing all that, L-Lysine really seems to be “beauty queen of essential amino acids”

5 Recommendation and posology

According to EFSA (European Food Safety Administration)[18], L-Lysine supplements are reported as safe for administration. The recommended daily intake of L-Lysine can vary depending on a person’s age, sex, weight, and activity level.

L-Lysine supplements are available in various forms, including powders, capsules, and tablets. The dosage and form of L-Lysine supplementation can vary depending on the individual’s needs and goals. In general, L-Lysine intake in the Western human diet is in the range 40-180 mg/kg/day. An upper limit of 300-400 mg/kg/day can be considered in humans[19].

Adverse events are mainly gastrointestinal symptoms; however, the risk incidence of gastrointestinal symptoms is very low. The “safety zone” of L-Lysine administration in healthy humans is below 6.0 g/d [20].

It is important to note that L-Lysine supplements should not be used as a substitute for a healthy diet. It is best to consume L-Lysine as part of a well-balanced diet that includes all the 9 essential amino acids, as formulated in Thot Aminos, together with whole and healthy foods.

In general, a dose of 30-60 mg/kg/day (or an average of 2-3 g/day) of L-lysine is sufficient. The upper limit is around 80 mg/kg/day. Doses may be adjusted as needed according to the following recommendations:

Adults:

Easy physical activity / adult 70 kg: 2-3/day (30 mg/kg x d^-1)
Moderate physical activity/ adult 70 kg: 3-4/day (45 mg/kg x d^-1)
Hard physical activity / adult 70 kg: 4-5/day (60 mg/kg x d^-1)

Teenagers:

Easy physical activity / teenager 43 kg: 1-2/day (35 mg/kg x d^-1)
Easy physical activity / teenager 63 kg: 1-2/day (33 mg/kg x d^-1)

Recommended doses of essential amino acids for adults (WHO/FAO/UNU, 2007)

Aminoacid	mg/kg x d^-1	pe adult 70 kg
Leucine	39.00	2,730.00
Valine	26.00	1,820.00
Izoleucine	20.00	1,400.00
Lysine	30.00	2,100.00
phenilalanine + Tyrosine	25.00	1,750.00
Threonine	15.00	1,050.00
Methionine + Cysteine	10.40	728.00
Histidine	10.00	700.00
Triptofan	4.00

Recommended doses for essential amino acids infants, children and adolescents (WHO/FAO/UNU, 2007)

	0.5 ani		1-2 ani		3-10 ani		11-14 ani		15-18 ani
Aminoacid	mg/kg x d^-1	pe copil 5 kg	mg/kg x d^-1	pe copil 12 kg	mg/kg x d^-1	pe copil 23 kg	mg/kg x d^-1	pe copil 43 kg	mg/kg x d^-1	pe copil 61 kg
Leucine	73.00	365.00	54.00	648.00	44.00	1,012.00	44.00	1,892.00	42.00	2,562.00
Valine	49.00	245.00	36.00	432.00	29.00	667.00	29.00	1,247.00	28.00	1,708.00
Izoleucine	36.00	180.00	27.00	324.00	23.00	529.00	22.00	946.00	21.00	1,281.00
Lysine	64.00	320.00	45.00	540.00	35.00	805.00	35.00	1,505.00	33.00	2,013.00
phenilalanine + Tyrosine	59.00	295.00	40.00	480.00	30.00	690.00	30.00	1,290.00	28.00	1,708.00
Threonine	34.00	170.00	23.00	276.00	18.00	414.00	18.00	774.00	17.00	1,037.00
Methionine + Cysteine	31.00	155.00	22.00	264.00	18.00	414.00	17.00	731.00	16.00	976.00
Histidine	22.00	110.00	15.00	180.00	12.00	276.00	12.00	516.00	11.00	671.00
Triptofan	9.50	47.50	6.40	76.80	4.80	110.40	4.80	206.40	4.50	274.50

*L-Lysine is more effectively used if taken on an empty stomach, at least 30 minutes away from meals or snacks. It does not require digestion, only dissolution. It has a very small size and is absorbed directly into the blood, is orthomolecular and does not require metabolism, being ready for use. **Essential amino acids have NO contraindications and L-lysine is recognized at European level by EFSA as safe for administration. At very high doses (above 6 g/day), discomfort in the digestive system may occur. The body does not produce them, they need to be taken from the outside and we need them all our lives. L-Lysine is an essential amino acid. For other recommendations, consult your doctor or therapist.

Thank you for taking time to read this article!

If you have any questions, please feel free to contact us:

Mail: office@thotnutrition.com

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[4] Yamauchi M, Terajima M, Shiiba M. Lysine Hydroxylation and Cross-Linking of Collagen. Methods Mol Biol. 2019;1934:309-324.

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